Detailed information of TA system TA043864

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 52366..52967
• Replicon: plasmid pEC361_3
• Accession: NZ_AP027414
• Organism: Escherichia coli strain EC361 isolate EC361

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: QUF17_RS01090
• Protein ID: WP_286338121.1
• Coordinates: 52587..52967 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: QUF17_RS01085
• Protein ID: WP_001190712.1
• Coordinates: 52366..52587 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF17_RS01045 (MUTS5_01990)	47838..48791	+	954	WP_286338118.1	DUF968 domain-containing protein	-
QUF17_RS01050 (MUTS5_02000)	48808..49530	+	723	WP_086351783.1	hypothetical protein	-
QUF17_RS01055 (MUTS5_02010)	49523..49789	+	267	WP_086351782.1	hypothetical protein	-
QUF17_RS01060 (MUTS5_02020)	49776..50528	+	753	WP_086351781.1	ead/Ea22-like family protein	-
QUF17_RS01065	50881..51114	+	234	WP_286338119.1	DUF4014 family protein	-
QUF17_RS01070 (MUTS5_02030)	51177..51428	+	252	WP_001283837.1	DNA polymerase III subunit theta	-
QUF17_RS01075 (MUTS5_02040)	51453..51806	+	354	WP_286338120.1	hypothetical protein	-
QUF17_RS01080 (MUTS5_02050)	51904..52293	+	390	WP_086351777.1	S24 family peptidase	-
QUF17_RS01085 (MUTS5_02060)	52366..52587	+	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
QUF17_RS01090 (MUTS5_02070)	52587..52967	+	381	WP_286338121.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
QUF17_RS01095 (MUTS5_02080)	53003..54103	-	1101	WP_044868365.1	hypothetical protein	-
QUF17_RS01100 (MUTS5_02090)	54090..54896	-	807	WP_261629111.1	hypothetical protein	-
QUF17_RS01105 (MUTS5_02100)	54903..55553	-	651	WP_079916754.1	DUF2829 domain-containing protein	-
QUF17_RS01110 (MUTS5_02110)	55568..56062	-	495	WP_068862852.1	dUTP diphosphatase	-
QUF17_RS01115 (MUTS5_02120)	56059..56547	-	489	WP_016262423.1	hypothetical protein	-
QUF17_RS01120 (MUTS5_02130)	56544..56888	-	345	WP_016262424.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..90284	90284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-122)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13707.63 Da
• Isoelectric Point: 5.6406

>T43864 WP_286338121.1 NZ_AP027414:52587-52967 [Escherichia coli]
MRHISPEELIVLHDANISRYGGLPGMSDPGRAEAIIGRVQARVVYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGIQVYDSPVLVELAVGAATGEIPVSSVAEKLRELYGSNI

Nucleotide

• Length: 381 bp

>T43864 NZ_AP027414:52587-52967 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGTGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCAACCTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGCTATTTCTACGCCGTAACGGTATACAGGTATATGATTCTCCCGTGCTGGTGGAACTTGCGGTGGGGGCTGCAACTGG
TGAAATCCCCGTATCTTCAGTAGCGGAAAAACTACGTGAACTATATGGTTCCAATATTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT43864 WP_001190712.1 NZ_AP027414:52366-52587 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT43864 NZ_AP027414:52366-52587 [Escherichia coli]
ATGCAATCCATTAACTTCCGAACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CJB6