Detailed information of TA system TA043862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TsxAB/Xre(antitoxin)
• Location: 6122..6623
• Replicon: plasmid pEC361_4
• Accession: NZ_AP027413
• Organism: Escherichia coli strain EC361 isolate EC361

Toxin (Protein)

• Gene name: TsxA
• Uniprot ID: H1ZXU8
• Locus tag: QUF17_RS00430
• Protein ID: WP_000702247.1
• Coordinates: 6122..6382 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: TsxB
• Uniprot ID: -
• Locus tag: QUF17_RS00435
• Protein ID: WP_001132407.1
• Coordinates: 6372..6623 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF17_RS00385	1730..1920	+	191	Protein_1	hypothetical protein	-
QUF17_RS00390 (MUTS5_00700)	2570..2722	+	153	Protein_2	DUF5431 family protein	-
QUF17_RS00395	2667..2789	+	123	WP_223170659.1	type I toxin-antitoxin system Hok family toxin	-
QUF17_RS00400 (MUTS5_00710)	2939..3535	+	597	WP_000125165.1	ProQ/FINO family protein	-
QUF17_RS00405 (MUTS5_00720)	3553..3837	-	285	WP_001162371.1	hypothetical protein	-
QUF17_RS00410 (MUTS5_00730)	3911..4576	-	666	WP_000532148.1	division plane positioning ATPase MipZ	-
QUF17_RS00415 (MUTS5_00740)	5175..5498	+	324	WP_000427403.1	hypothetical protein	-
QUF17_RS00420 (MUTS5_00750)	5540..5749	+	210	WP_000866036.1	hypothetical protein	-
QUF17_RS00425 (MUTS5_00760)	5846..6070	+	225	WP_000814381.1	hypothetical protein	-
QUF17_RS00430 (MUTS5_00770)	6122..6382	+	261	WP_000702247.1	hypothetical protein	Toxin
QUF17_RS00435 (MUTS5_00780)	6372..6623	+	252	WP_001132407.1	transcriptional regulator	Antitoxin
QUF17_RS00440 (MUTS5_00790)	7173..7451	+	279	WP_000545938.1	hypothetical protein	-
QUF17_RS00445 (MUTS5_00810)	7838..8281	-	444	WP_000192626.1	hypothetical protein	-
QUF17_RS00450 (MUTS5_00820)	8578..8760	+	183	WP_000833470.1	type II toxin-antitoxin system HicA family toxin	-
QUF17_RS00455 (MUTS5_00830)	8785..9222	+	438	WP_000466318.1	type II toxin-antitoxin system HicB family antitoxin	-
QUF17_RS00460 (MUTS5_00840)	9353..9673	-	321	WP_021580489.1	hypothetical protein	-
QUF17_RS00465 (MUTS5_00850)	9677..9964	-	288	WP_000212745.1	hypothetical protein	-
QUF17_RS00470 (MUTS5_00860)	9966..10457	-	492	WP_001167293.1	hypothetical protein	-
QUF17_RS00475	10766..10906	-	141	Protein_19	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..58371	58371

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (8-37)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9817.29 Da
• Isoelectric Point: 10.0665

>T43862 WP_000702247.1 NZ_AP027413:6122-6382 [Escherichia coli]
MKIRKGDRQYYLNKEGDTFHLVKRVKTFSKSATLGKTKATVKTVADLVFHEKAFDTIDFASDGLRENDKEIVSMMIQEMS
EGKNAK

Nucleotide

• Length: 261 bp

>T43862 NZ_AP027413:6122-6382 [Escherichia coli]
ATGAAGATAAGAAAAGGTGACAGACAATATTATCTGAATAAAGAAGGTGATACGTTTCATCTGGTGAAGCGAGTAAAAAC
TTTTTCTAAGTCTGCGACTTTAGGAAAAACGAAAGCAACGGTGAAAACTGTTGCCGATCTTGTTTTTCATGAGAAGGCAT
TCGACACTATCGACTTTGCCAGCGACGGATTAAGAGAGAATGATAAAGAAATTGTTTCTATGATGATTCAGGAAATGAGT
GAGGGAAAAAATGCCAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9404.77 Da
• Isoelectric Point: 9.8120

>AT43862 WP_001132407.1 NZ_AP027413:6372-6623 [Escherichia coli]
MPNENTPENIKRLRQKIGLTQKECAEIFSMSPRTWRRKEEPVGTASGTALTPVEFKFLLLLAGEHPDYVLCKRNKKNSDS
GSN

Nucleotide

• Length: 252 bp

>AT43862 NZ_AP027413:6372-6623 [Escherichia coli]
ATGCCAAATGAAAACACACCAGAAAACATTAAACGTCTGCGGCAAAAAATTGGCCTGACACAGAAAGAATGTGCCGAAAT
ATTTAGCATGAGTCCGCGTACCTGGCGTCGCAAAGAAGAACCTGTCGGAACTGCAAGCGGTACAGCACTTACACCAGTAG
AGTTTAAATTTCTTCTGCTTTTAGCTGGAGAACACCCTGATTATGTACTTTGTAAAAGGAATAAAAAGAATTCTGATTCT
GGAAGTAATTAA

Structures

Toxin

• AlphaFold DB: A0A1P8DNC0