Detailed information of TA system TA043848

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3096701..3097536
• Replicon: chromosome
• Accession: NZ_AP027411
• Organism: Escherichia coli strain EC521 isolate EC521

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A0J5ZPW7
• Locus tag: QUE69_RS15620
• Protein ID: WP_000854722.1
• Coordinates: 3096701..3097078 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8S7U9D1
• Locus tag: QUE69_RS15625
• Protein ID: WP_024168534.1
• Coordinates: 3097168..3097536 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE69_RS15595 (3092082)	3092082..3094334	-	2253	Protein_2881	alpha-amylase family glycosyl hydrolase	-
QUE69_RS15605 (3095069)	3095069..3095914	-	846	WP_001280434.1	DUF4942 domain-containing protein	-
QUE69_RS15610 (3095999)	3095999..3096196	-	198	WP_000445281.1	DUF957 domain-containing protein	-
QUE69_RS15615 (3096216)	3096216..3096704	-	489	WP_000761669.1	DUF5983 family protein	-
QUE69_RS15620 (3096701)	3096701..3097078	-	378	WP_000854722.1	TA system toxin CbtA family protein	Toxin
QUE69_RS15625 (3097168)	3097168..3097536	-	369	WP_024168534.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
QUE69_RS15630 (3097616)	3097616..3097837	-	222	WP_000692350.1	DUF987 domain-containing protein	-
QUE69_RS15635 (3097924)	3097924..3098004	-	81	Protein_2888	hypothetical protein	-
QUE69_RS15640 (3097981)	3097981..3099192	-	1212	Protein_2889	dynamin family protein	-
QUE69_RS15645 (3099303)	3099303..3102152	-	2850	WP_089637179.1	calcium-binding autotransporter Cah	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14103.15 Da
• Isoelectric Point: 8.2904

>T43848 WP_000854722.1 NZ_AP027411:c3097078-3096701 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQILLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T43848 NZ_AP027411:c3097078-3096701 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACCGACCAGCCGGGGTTC
AGCACCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCTTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13622.44 Da
• Isoelectric Point: 6.3159

>AT43848 WP_024168534.1 NZ_AP027411:c3097536-3097168 [Escherichia coli]
VSDILPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT43848 NZ_AP027411:c3097536-3097168 [Escherichia coli]
GTGTCAGACATACTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0J5ZPW7