Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2786805..2787459 Replicon chromosome
Accession NZ_AP027411
Organism Escherichia coli strain EC521 isolate EC521

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag QUE69_RS14065 Protein ID WP_000244777.1
Coordinates 2787052..2787459 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUE69_RS14060 Protein ID WP_000354046.1
Coordinates 2786805..2787071 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE69_RS14035 (2781974) 2781974..2782717 + 744 WP_000951964.1 SDR family oxidoreductase -
QUE69_RS14040 (2782774) 2782774..2784207 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
QUE69_RS14045 (2784252) 2784252..2784563 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
QUE69_RS14050 (2784727) 2784727..2785386 + 660 WP_000250274.1 hemolysin III family protein -
QUE69_RS14055 (2785582) 2785582..2786562 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
QUE69_RS14060 (2786805) 2786805..2787071 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUE69_RS14065 (2787052) 2787052..2787459 + 408 WP_000244777.1 protein YgfX Toxin
QUE69_RS14070 (2787499) 2787499..2788020 - 522 WP_001055874.1 flavodoxin FldB -
QUE69_RS14075 (2788132) 2788132..2789028 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUE69_RS14080 (2789053) 2789053..2789745 + 693 WP_213831663.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUE69_RS14085 (2789769) 2789769..2791502 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T43846 WP_000244777.1 NZ_AP027411:2787052-2787459 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T43846 NZ_AP027411:2787052-2787459 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT43846 WP_000354046.1 NZ_AP027411:2786805-2787071 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT43846 NZ_AP027411:2786805-2787071 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References