Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4545342..4546177 Replicon chromosome
Accession NZ_AP027408
Organism Escherichia coli strain EC507 isolate EC507

Toxin (Protein)


Gene name yeeV Uniprot ID A0A7W4PV54
Locus tag QUE45_RS23545 Protein ID WP_000854821.1
Coordinates 4545800..4546177 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID F4TIK7
Locus tag QUE45_RS23540 Protein ID WP_001285605.1
Coordinates 4545342..4545710 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE45_RS23515 (4542752) 4542752..4542997 - 246 Protein_4386 IS91 family transposase -
QUE45_RS23520 (4543166) 4543166..4544328 + 1163 WP_085947771.1 IS3-like element IS3 family transposase -
QUE45_RS23525 (4544361) 4544361..4544462 + 102 Protein_4388 antirestriction protein -
QUE45_RS23530 (4544478) 4544478..4544954 + 477 WP_004025728.1 RadC family protein -
QUE45_RS23535 (4545041) 4545041..4545262 + 222 Protein_4390 DUF987 domain-containing protein -
QUE45_RS23540 (4545342) 4545342..4545710 + 369 WP_001285605.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
QUE45_RS23545 (4545800) 4545800..4546177 + 378 WP_000854821.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
QUE45_RS23550 (4546174) 4546174..4546323 + 150 Protein_4393 DUF5983 family protein -
QUE45_RS23555 (4546399) 4546399..4546596 + 198 WP_086795284.1 DUF957 domain-containing protein -
QUE45_RS23560 (4546681) 4546681..4547523 + 843 WP_001696593.1 DUF4942 domain-containing protein -
QUE45_RS23565 (4547760) 4547760..4547921 + 162 Protein_4396 RhuM family protein -
QUE45_RS23570 (4548318) 4548318..4549502 + 1185 WP_001172876.1 sugar efflux transporter -
QUE45_RS23575 (4549613) 4549613..4550536 + 924 WP_000535963.1 carboxylate/amino acid/amine transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13930.95 Da        Isoelectric Point: 8.5163

>T43838 WP_000854821.1 NZ_AP027408:4545800-4546177 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Download         Length: 378 bp

>T43838 NZ_AP027408:4545800-4546177 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13652.48 Da        Isoelectric Point: 6.0601

>AT43838 WP_001285605.1 NZ_AP027408:4545342-4545710 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRACIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Download         Length: 369 bp

>AT43838 NZ_AP027408:4545342-4545710 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCTGTATTAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7W4PV54

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2V3N6

References