Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3654161..3654815 Replicon chromosome
Accession NZ_AP027408
Organism Escherichia coli strain EC507 isolate EC507

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag QUE45_RS19200 Protein ID WP_000244781.1
Coordinates 3654161..3654568 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUE45_RS19205 Protein ID WP_000354046.1
Coordinates 3654549..3654815 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE45_RS19180 (3650118) 3650118..3651851 - 1734 WP_124037221.1 single-stranded-DNA-specific exonuclease RecJ -
QUE45_RS19185 (3651857) 3651857..3652567 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUE45_RS19190 (3652592) 3652592..3653488 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUE45_RS19195 (3653600) 3653600..3654121 + 522 WP_001055874.1 flavodoxin FldB -
QUE45_RS19200 (3654161) 3654161..3654568 - 408 WP_000244781.1 protein YgfX Toxin
QUE45_RS19205 (3654549) 3654549..3654815 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUE45_RS19210 (3655058) 3655058..3656038 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
QUE45_RS19215 (3656234) 3656234..3656893 - 660 WP_000250274.1 hemolysin III family protein -
QUE45_RS19220 (3657057) 3657057..3657368 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
QUE45_RS19225 (3657413) 3657413..3658846 + 1434 WP_032278899.1 6-phospho-beta-glucosidase BglA -
QUE45_RS19230 (3658903) 3658903..3659646 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T43833 WP_000244781.1 NZ_AP027408:c3654568-3654161 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T43833 NZ_AP027408:c3654568-3654161 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCTTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT43833 WP_000354046.1 NZ_AP027408:c3654815-3654549 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT43833 NZ_AP027408:c3654815-3654549 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References