Detailed information of TA system TA043822

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1950474..1951111
• Replicon: chromosome
• Accession: NZ_AP027408
• Organism: Escherichia coli strain EC507 isolate EC507

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: QUE45_RS10670
• Protein ID: WP_000813794.1
• Coordinates: 1950474..1950650 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QUE45_RS10675
• Protein ID: WP_113368043.1
• Coordinates: 1950695..1951111 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE45_RS10650 (1946093)	1946093..1947268	-	1176	WP_250147805.1	BenE family transporter YdcO	-
QUE45_RS10655 (1947360)	1947360..1947896	+	537	WP_000429150.1	DNA-binding transcriptional regulator SutR	-
QUE45_RS10660 (1947969)	1947969..1949930	+	1962	WP_001383134.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QUE45_RS10665 (1950022)	1950022..1950252	-	231	WP_000494244.1	YncJ family protein	-
QUE45_RS10670 (1950474)	1950474..1950650	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QUE45_RS10675 (1950695)	1950695..1951111	+	417	WP_113368043.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QUE45_RS10680 (1951190)	1951190..1952596	+	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
QUE45_RS10685 (1952841)	1952841..1953986	+	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
QUE45_RS10690 (1954004)	1954004..1955017	+	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
QUE45_RS10695 (1955018)	1955018..1955959	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-67)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T43822 WP_000813794.1 NZ_AP027408:1950474-1950650 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T43822 NZ_AP027408:1950474-1950650 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15278.69 Da
• Isoelectric Point: 4.7386

>AT43822 WP_113368043.1 NZ_AP027408:1950695-1951111 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLMMV

Nucleotide

• Length: 417 bp

>AT43822 NZ_AP027408:1950695-1951111 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGATGATGGTTTAA

Structures

Toxin

• PDB: 6HPB