Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 4882406..4883238 Replicon chromosome
Accession NZ_AP027404
Organism Escherichia coli strain EC416 isolate EC416

Toxin (Protein)


Gene name yeeV Uniprot ID S1PD64
Locus tag QUE37_RS25015 Protein ID WP_000854753.1
Coordinates 4882406..4882780 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID V0ULY5
Locus tag QUE37_RS25020 Protein ID WP_001315620.1
Coordinates 4882870..4883238 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE37_RS24975 (4877621) 4877621..4878727 + 1107 WP_032185902.1 N-acetylneuraminate epimerase -
QUE37_RS24980 (4878792) 4878792..4879772 + 981 WP_001295601.1 sialate O-acetylesterase -
QUE37_RS24985 (4879780) 4879780..4880430 - 651 WP_001037966.1 HNH endonuclease -
QUE37_RS24990 (4880567) 4880567..4880710 + 144 Protein_4686 HNH endonuclease -
QUE37_RS24995 (4880809) 4880809..4880994 + 186 WP_000066585.1 hypothetical protein -
QUE37_RS25000 (4881478) 4881478..4881627 - 150 Protein_4688 hypothetical protein -
QUE37_RS25005 (4881712) 4881712..4881909 - 198 WP_000839282.1 DUF957 domain-containing protein -
QUE37_RS25010 (4881921) 4881921..4882409 - 489 WP_000777548.1 DUF5983 family protein -
QUE37_RS25015 (4882406) 4882406..4882780 - 375 WP_000854753.1 TA system toxin CbtA family protein Toxin
QUE37_RS25020 (4882870) 4882870..4883238 - 369 WP_001315620.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
QUE37_RS25025 (4883401) 4883401..4883622 - 222 WP_000692345.1 DUF987 domain-containing protein -
QUE37_RS25030 (4883685) 4883685..4884161 - 477 WP_001186711.1 RadC family protein -
QUE37_RS25035 (4884177) 4884177..4884662 - 486 WP_000849588.1 antirestriction protein -
QUE37_RS25040 (4884717) 4884717..4885535 - 819 WP_001234620.1 DUF932 domain-containing protein -
QUE37_RS25045 (4885635) 4885635..4885868 - 234 WP_001119729.1 DUF905 family protein -
QUE37_RS25050 (4885947) 4885947..4886402 - 456 WP_000581504.1 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14005.00 Da        Isoelectric Point: 8.2905

>T43806 WP_000854753.1 NZ_AP027404:c4882780-4882406 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEKR

Download         Length: 375 bp

>T43806 NZ_AP027404:c4882780-4882406 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13474.20 Da        Isoelectric Point: 6.2050

>AT43806 WP_001315620.1 NZ_AP027404:c4883238-4882870 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT43806 NZ_AP027404:c4883238-4882870 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGTCTGACCTGCAAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LVU0

Antitoxin

Source ID Structure
AlphaFold DB V0ULY5

References