Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4846137..4846713 Replicon chromosome
Accession NZ_AP027404
Organism Escherichia coli strain EC416 isolate EC416

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag QUE37_RS24815 Protein ID WP_001297643.1
Coordinates 4846426..4846713 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A829G8Z0
Locus tag QUE37_RS24810 Protein ID WP_000063156.1
Coordinates 4846137..4846439 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE37_RS24790 (4841164) 4841164..4842384 + 1221 WP_000873752.1 amino acid deaminase -
QUE37_RS24795 (4842652) 4842652..4844802 + 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
QUE37_RS24800 (4844933) 4844933..4845136 + 204 WP_000467859.1 YbdD/YjiX family protein -
QUE37_RS24805 (4845147) 4845147..4846103 + 957 WP_001312515.1 GTPase -
QUE37_RS24810 (4846137) 4846137..4846439 - 303 WP_000063156.1 BrnA antitoxin family protein Antitoxin
QUE37_RS24815 (4846426) 4846426..4846713 - 288 WP_001297643.1 BrnT family toxin Toxin
QUE37_RS24820 (4846912) 4846912..4847608 - 697 Protein_4652 IS1 family transposase -
QUE37_RS24825 (4847934) 4847934..4849166 + 1233 WP_032185894.1 multidrug efflux MFS transporter MdtM -
QUE37_RS24830 (4849207) 4849207..4850487 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4846912..4847121 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T43805 WP_001297643.1 NZ_AP027404:c4846713-4846426 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T43805 NZ_AP027404:c4846713-4846426 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.99 Da        Isoelectric Point: 9.4646

>AT43805 WP_000063156.1 NZ_AP027404:c4846439-4846137 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT43805 NZ_AP027404:c4846439-4846137 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A829G8Z0

References