Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4241926..4242544 Replicon chromosome
Accession NZ_AP027404
Organism Escherichia coli strain EC416 isolate EC416

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QUE37_RS21820 Protein ID WP_001291435.1
Coordinates 4242326..4242544 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QUE37_RS21815 Protein ID WP_000344800.1
Coordinates 4241926..4242300 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE37_RS21805 (4237015) 4237015..4238208 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
QUE37_RS21810 (4238231) 4238231..4241380 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
QUE37_RS21815 (4241926) 4241926..4242300 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QUE37_RS21820 (4242326) 4242326..4242544 + 219 WP_001291435.1 HHA domain-containing protein Toxin
QUE37_RS21825 (4242716) 4242716..4243267 + 552 WP_000102564.1 maltose O-acetyltransferase -
QUE37_RS21830 (4243383) 4243383..4243853 + 471 WP_000136192.1 YlaC family protein -
QUE37_RS21835 (4244017) 4244017..4245567 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QUE37_RS21840 (4245609) 4245609..4245962 - 354 WP_000878140.1 DUF1428 domain-containing protein -
QUE37_RS21850 (4246341) 4246341..4246652 + 312 WP_000409911.1 MGMT family protein -
QUE37_RS21855 (4246683) 4246683..4247255 - 573 WP_089563984.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T43802 WP_001291435.1 NZ_AP027404:4242326-4242544 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T43802 NZ_AP027404:4242326-4242544 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT43802 WP_000344800.1 NZ_AP027404:4241926-4242300 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT43802 NZ_AP027404:4241926-4242300 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References