Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1499354..1500008 Replicon chromosome
Accession NZ_AP027404
Organism Escherichia coli strain EC416 isolate EC416

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag QUE37_RS08200 Protein ID WP_000244777.1
Coordinates 1499601..1500008 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUE37_RS08195 Protein ID WP_000354046.1
Coordinates 1499354..1499620 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE37_RS08170 (1494523) 1494523..1495266 + 744 WP_000951964.1 SDR family oxidoreductase -
QUE37_RS08175 (1495323) 1495323..1496756 - 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
QUE37_RS08180 (1496801) 1496801..1497112 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
QUE37_RS08185 (1497276) 1497276..1497935 + 660 WP_000250274.1 hemolysin III family protein -
QUE37_RS08190 (1498131) 1498131..1499111 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
QUE37_RS08195 (1499354) 1499354..1499620 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUE37_RS08200 (1499601) 1499601..1500008 + 408 WP_000244777.1 protein YgfX Toxin
QUE37_RS08205 (1500048) 1500048..1500569 - 522 WP_001055874.1 flavodoxin FldB -
QUE37_RS08210 (1500681) 1500681..1501577 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUE37_RS08215 (1501602) 1501602..1502312 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUE37_RS08220 (1502318) 1502318..1504051 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T43790 WP_000244777.1 NZ_AP027404:1499601-1500008 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T43790 NZ_AP027404:1499601-1500008 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT43790 WP_000354046.1 NZ_AP027404:1499354-1499620 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT43790 NZ_AP027404:1499354-1499620 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References