Detailed information of TA system TA043788

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1308417..1309252
• Replicon: chromosome
• Accession: NZ_AP027404
• Organism: Escherichia coli strain EC416 isolate EC416

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A1V3VXN0
• Locus tag: QUE37_RS07240
• Protein ID: WP_000854801.1
• Coordinates: 1308875..1309252 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1V3VXQ4
• Locus tag: QUE37_RS07235
• Protein ID: WP_001360188.1
• Coordinates: 1308417..1308785 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE37_RS07210 (1305532)	1305532..1306350	+	819	WP_001234702.1	DUF932 domain-containing protein	-
QUE37_RS07215 (1306441)	1306441..1306926	+	486	WP_000214418.1	antirestriction protein	-
QUE37_RS07220 (1306942)	1306942..1307418	+	477	WP_001186725.1	RadC family protein	-
QUE37_RS07225 (1307487)	1307487..1307708	+	222	WP_000692334.1	DUF987 domain-containing protein	-
QUE37_RS07230 (1307723)	1307723..1308367	+	645	WP_048949123.1	hypothetical protein	-
QUE37_RS07235 (1308417)	1308417..1308785	+	369	WP_001360188.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
QUE37_RS07240 (1308875)	1308875..1309252	+	378	WP_000854801.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
QUE37_RS07245 (1309249)	1309249..1309737	+	489	WP_247806646.1	DUF5983 family protein	-
QUE37_RS07250 (1309749)	1309749..1309946	+	198	WP_000839269.1	DUF957 domain-containing protein	-
QUE37_RS07255 (1310031)	1310031..1310876	+	846	WP_044325567.1	DUF4942 domain-containing protein	-
QUE37_RS07265 (1311176)	1311176..1311682	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
QUE37_RS07270 (1311761)	1311761..1313602	-	1842	WP_000437375.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13881.87 Da
• Isoelectric Point: 7.3219

>T43788 WP_000854801.1 NZ_AP027404:1308875-1309252 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSQLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T43788 NZ_AP027404:1308875-1309252 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCAACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13563.27 Da
• Isoelectric Point: 6.2044

>AT43788 WP_001360188.1 NZ_AP027404:1308417-1308785 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGVTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT43788 NZ_AP027404:1308417-1308785 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCATGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGGTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A1V3VXN0

Antitoxin

• AlphaFold DB: A0A1V3VXQ4