Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 1774..2321 Replicon plasmid pEC416_1
Accession NZ_AP027403
Organism Escherichia coli strain EC416 isolate EC416

Toxin (Protein)


Gene name parE Uniprot ID G7QEJ9
Locus tag QUE37_RS00405 Protein ID WP_001384452.1
Coordinates 2043..2321 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A7B6MRK1
Locus tag QUE37_RS00400 Protein ID WP_032191903.1
Coordinates 1774..2043 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE37_RS00390 (1) 1..858 + 858 WP_032191900.1 incFII family plasmid replication initiator RepA -
QUE37_RS00395 (1561) 1561..1719 + 159 WP_032191902.1 hypothetical protein -
QUE37_RS00400 (1774) 1774..2043 + 270 WP_032191903.1 type II toxin-antitoxin system antitoxin YacA Antitoxin
QUE37_RS00405 (2043) 2043..2321 + 279 WP_001384452.1 type II toxin-antitoxin system toxin YacB Toxin
QUE37_RS00410 (3112) 3112..3809 + 698 WP_249824342.1 IS1-like element IS1A family transposase -
QUE37_RS00415 (4111) 4111..4638 + 528 WP_137531647.1 YopT-type cysteine protease domain-containing protein -
QUE37_RS00420 (4962) 4962..5165 + 204 WP_286347729.1 recombinase family protein -
QUE37_RS00425 (5346) 5346..5906 - 561 Protein_7 IS91-like element IS91 family transposase -
QUE37_RS00430 (6098) 6098..7285 - 1188 WP_000937612.1 IS91-like element IS91 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..98045 98045


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10762.38 Da        Isoelectric Point: 8.4615

>T43781 WP_001384452.1 NZ_AP027403:2043-2321 [Escherichia coli]
MEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVY
ILRVLHTAQKWP

Download         Length: 279 bp

>T43781 NZ_AP027403:2043-2321 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACCGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGAATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10152.51 Da        Isoelectric Point: 10.2879

>AT43781 WP_032191903.1 NZ_AP027403:1774-2043 [Escherichia coli]
MTQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVAAGRQQLGRGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT43781 NZ_AP027403:1774-2043 [Escherichia coli]
ATGACACAGGTTAACATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGGGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CL36

Antitoxin

Source ID Structure
AlphaFold DB A0A7B6MRK1

References