Detailed information of TA system TA043765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 13279..13850
• Replicon: plasmid N4E_p1
• Accession: NZ_AP027303
• Organism: Enterococcus faecalis strain N4E

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A2Z2CGM4
• Locus tag: QUD80_RS14305
• Protein ID: WP_002362432.1
• Coordinates: 13279..13620 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3KHK9
• Locus tag: QUD80_RS14310
• Protein ID: WP_002362431.1
• Coordinates: 13620..13850 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD80_RS14285 (N4E_28170)	8780..9628	+	849	WP_001258486.1	streptomycin adenylyltransferase Str	-
QUD80_RS14295 (N4E_28200)	11362..11964	-	603	WP_002362434.1	Fic family protein	-
QUD80_RS14300 (N4E_28210)	12229..13167	-	939	WP_174115147.1	hypothetical protein	-
QUD80_RS14305 (N4E_28220)	13279..13620	-	342	WP_002362432.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
QUD80_RS14310 (N4E_28230)	13620..13850	-	231	WP_002362431.1	hypothetical protein	Antitoxin
QUD80_RS14315 (N4E_28240)	14054..14674	+	621	WP_002367784.1	recombinase family protein	-
QUD80_RS14320 (N4E_28250)	14664..14978	+	315	WP_002367785.1	hypothetical protein	-
QUD80_RS14325 (N4E_28260)	14972..15178	+	207	WP_002367786.1	hypothetical protein	-
QUD80_RS14330 (N4E_28270)	15338..15532	+	195	WP_002367787.1	hypothetical protein	-
QUD80_RS14335 (N4E_28280)	15544..15735	+	192	WP_002367788.1	hypothetical protein	-
QUD80_RS14340 (N4E_28290)	15905..16120	+	216	WP_002367791.1	leucocin A/sakacin P family class II bacteriocin	-
QUD80_RS14345 (N4E_28300)	16121..16462	+	342	WP_002367792.1	bacteriocin immunity protein	-
QUD80_RS14350 (N4E_28310)	16878..17396	+	519	WP_002367793.1	hypothetical protein	-
QUD80_RS14355 (N4E_28320)	17344..17559	+	216	WP_002415356.1	hypothetical protein	-
QUD80_RS14360	17651..17737	+	87	WP_012881081.1	type I toxin-antitoxin system Fst family toxin	-
QUD80_RS14365 (N4E_28330)	17994..18290	+	297	WP_002367795.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	str / erm(B) / cat / tet(L) / tet(M)	-	1..51671	51671

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13244.44 Da
• Isoelectric Point: 8.0113

>T43765 WP_002362432.1 NZ_AP027303:c13620-13279 [Enterococcus faecalis]
MSDEKKYIPKKGDIVWIDFDPSAEKEIQKRRPGLVVSRYEFNRKTLFAVICPITSTIKNMPTRYTLPDEMETHGQVVISQ
LKSLDFAERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T43765 NZ_AP027303:c13620-13279 [Enterococcus faecalis]
ATGAGTGACGAAAAGAAATATATTCCTAAAAAGGGCGATATTGTATGGATAGATTTCGATCCTTCAGCTGAAAAAGAAAT
ACAGAAAAGACGTCCAGGTCTAGTAGTTTCTAGATATGAGTTTAATAGAAAGACATTGTTTGCAGTAATTTGTCCCATAA
CTTCTACAATTAAAAATATGCCAACTCGTTATACATTACCTGATGAAATGGAAACTCATGGACAAGTCGTTATTTCCCAA
TTAAAATCTTTAGACTTTGCAGAAAGAAAGTTAAGCCAAATAGAACATTTACCATTAAAGGATATGGCTAAAATAGATCA
AATCATTGAATACATTTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8510.42 Da
• Isoelectric Point: 4.3855

>AT43765 WP_002362431.1 NZ_AP027303:c13850-13620 [Enterococcus faecalis]
MLTTKSRKQGSSVVLTLPSNNGQKPKADQEYIVMYSDDGTITLVPKLQDPFSGGSEGEYYEKDEWHDIEPGGRELF

Nucleotide

• Length: 231 bp

>AT43765 NZ_AP027303:c13850-13620 [Enterococcus faecalis]
ATGTTAACCACAAAATCTAGAAAACAAGGAAGTTCTGTAGTATTGACATTACCATCTAATAACGGACAGAAACCAAAAGC
AGACCAAGAATATATAGTGATGTATTCTGATGATGGGACAATTACGTTAGTCCCTAAATTACAAGACCCATTTAGTGGTG
GATCAGAAGGGGAGTACTACGAAAAAGATGAATGGCATGACATTGAACCTGGAGGGAGAGAATTATTTTAA

Structures

Toxin

• AlphaFold DB: A0A2Z2CGM4

Antitoxin

• AlphaFold DB: R3KHK9