Detailed information of TA system TA043744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 861961..862532
• Replicon: chromosome
• Accession: NZ_AP027302
• Organism: Enterococcus faecalis strain N4E

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: QUD80_RS04160
• Protein ID: WP_174114068.1
• Coordinates: 862191..862532 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: R3JGB1
• Locus tag: QUD80_RS04155
• Protein ID: WP_002354773.1
• Coordinates: 861961..862191 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD80_RS04135 (857093)	857093..858709	-	1617	WP_174114069.1	phosphatase PAP2/LCP family protein	-
QUD80_RS04140 (859395)	859395..860033	+	639	WP_002378863.1	lytic polysaccharide monooxygenase	-
QUD80_RS04145 (860200)	860200..861192	-	993	WP_002378862.1	biotin--[acetyl-CoA-carboxylase] ligase	-
QUD80_RS04150 (861331)	861331..861546	+	216	WP_002354768.1	zinc ribbon domain-containing protein	-
QUD80_RS04155 (861961)	861961..862191	+	231	WP_002354773.1	hypothetical protein	Antitoxin
QUD80_RS04160 (862191)	862191..862532	+	342	WP_174114068.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
QUD80_RS04165 (862903)	862903..866517	+	3615	WP_002389470.1	DNA-directed RNA polymerase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13177.42 Da
• Isoelectric Point: 8.8678

>T43744 WP_174114068.1 NZ_AP027302:862191-862532 [Enterococcus faecalis]
MNVEKKYIPKKGDIVWIDFDPAAGKEIQKRRPGLVVSRYEFNRKTMFAVICPIISTIKNIPTRYTLPDDIETHGQVVISQ
LQSLDFTERKLSQIEHLPLKDMAKIDQIIEYIF

Nucleotide

• Length: 342 bp

>T43744 NZ_AP027302:862191-862532 [Enterococcus faecalis]
ATGAACGTTGAAAAGAAGTACATTCCCAAAAAAGGAGATATTGTATGGATTGATTTTGATCCTGCTGCAGGTAAAGAAAT
TCAGAAGAGACGCCCTGGTTTAGTCGTGTCAAGATATGAATTTAATCGGAAAACCATGTTTGCTGTGATTTGCCCGATTA
TTTCCACCATTAAAAATATTCCCACTCGATATACGTTGCCCGATGACATAGAAACCCATGGACAAGTAGTGATCTCACAA
CTGCAATCTTTGGACTTCACTGAAAGAAAATTAAGTCAAATTGAGCATTTGCCATTAAAAGATATGGCGAAAATTGACCA
AATAATTGAATATATTTTCTAG

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.67 Da
• Isoelectric Point: 4.3313

>AT43744 WP_002354773.1 NZ_AP027302:861961-862191 [Enterococcus faecalis]
MLTTKSRRQGSSVVLTLPTDNGKKPKVDQEYIVMYSDDGTITLVPKIQDPFSEGPEGEYYEKDEWHDLAPEGRELF

Nucleotide

• Length: 231 bp

>AT43744 NZ_AP027302:861961-862191 [Enterococcus faecalis]
ATGCTAACAACTAAATCAAGAAGACAAGGAAGCTCGGTCGTTTTAACTTTACCCACTGATAATGGTAAAAAACCAAAAGT
CGACCAAGAATATATTGTGATGTATTCTGACGACGGTACAATTACATTAGTCCCTAAAATTCAGGATCCATTTAGTGAAG
GCCCAGAAGGTGAGTATTATGAAAAAGATGAGTGGCATGATCTTGCACCTGAGGGGAGAGAACTTTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A0M2A812