Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2292095..2292666 Replicon chromosome
Accession NZ_AP027299
Organism Enterococcus hirae strain L6E

Toxin (Protein)


Gene name mazF Uniprot ID I6SW09
Locus tag QUD95_RS10595 Protein ID WP_010738536.1
Coordinates 2292095..2292436 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I6T4M0
Locus tag QUD95_RS10600 Protein ID WP_010738535.1
Coordinates 2292436..2292666 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUD95_RS10575 (L6E_20700) 2287319..2288320 + 1002 WP_034866081.1 aspartate-semialdehyde dehydrogenase -
QUD95_RS10580 (L6E_20710) 2288317..2289666 + 1350 WP_010720195.1 aspartate kinase -
QUD95_RS10585 (L6E_20720) 2289626..2290735 + 1110 WP_034866082.1 M20 family metallopeptidase -
QUD95_RS10590 (L6E_20730) 2290765..2291931 + 1167 WP_010738537.1 pyridoxal phosphate-dependent aminotransferase -
QUD95_RS10595 (L6E_20740) 2292095..2292436 - 342 WP_010738536.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QUD95_RS10600 (L6E_20750) 2292436..2292666 - 231 WP_010738535.1 hypothetical protein Antitoxin
QUD95_RS10605 (L6E_20760) 2293125..2294504 + 1380 WP_010738534.1 FAD-dependent oxidoreductase -
QUD95_RS10610 (L6E_20770) 2294685..2295650 - 966 WP_286335818.1 AraC family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-112)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13101.46 Da        Isoelectric Point: 10.1962

>T43726 WP_010738536.1 NZ_AP027299:c2292436-2292095 [Enterococcus hirae]
VPKARNYIPKKGDIVWIDFDPSTGKEIQKRRPGLVVSRYEFNCTTMFAIICPITSTIKNLPTRYSLPKDLDTKGQVLISQ
LKSLDFKERKLKKVENLPLQDMAKIDQIIQYIF

Download         Length: 342 bp

>T43726 NZ_AP027299:c2292436-2292095 [Enterococcus hirae]
GTGCCTAAAGCTAGAAATTATATTCCTAAAAAAGGGGATATTGTTTGGATCGATTTTGATCCATCCACAGGCAAAGAAAT
TCAAAAGAGAAGACCAGGCTTGGTTGTATCTCGTTATGAATTTAATTGCACAACAATGTTTGCAATAATCTGTCCAATTA
CTTCTACTATAAAAAATCTACCGACACGCTATTCGCTTCCCAAAGATTTAGATACAAAGGGACAAGTACTTATTTCTCAA
TTAAAATCACTTGATTTCAAGGAAAGAAAACTCAAAAAGGTCGAAAATTTACCTTTACAAGATATGGCTAAAATTGATCA
GATTATCCAGTATATATTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8577.57 Da        Isoelectric Point: 4.1196

>AT43726 WP_010738535.1 NZ_AP027299:c2292666-2292436 [Enterococcus hirae]
MLITKSRLQGSSVVITLPSDNGKKPSENQEYIVVYSEDGTITLVPKIEDPFSAGQEAEYYEKDEWKDLTPEGREIL

Download         Length: 231 bp

>AT43726 NZ_AP027299:c2292666-2292436 [Enterococcus hirae]
ATGCTCATTACAAAATCTAGATTACAAGGTAGTTCTGTAGTAATTACACTACCGTCTGATAATGGTAAAAAACCTTCAGA
AAATCAAGAATATATTGTTGTCTACTCTGAAGATGGGACAATCACATTAGTTCCTAAAATCGAAGATCCATTTAGTGCTG
GACAAGAAGCTGAGTATTACGAAAAAGATGAATGGAAAGACTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7Z9DJ27

Antitoxin

Source ID Structure
AlphaFold DB A0A449E900

References