Detailed information of TA system TA043696

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 61183..62291
• Replicon: plasmid K6D_p1
• Accession: NZ_AP027295
• Organism: Enterococcus faecium strain K6D

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A3S8RBH6
• Locus tag: QUD90_RS12895
• Protein ID: WP_071661597.1
• Coordinates: 61422..62291 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: QUD90_RS12890
• Protein ID: WP_000205227.1
• Coordinates: 61183..61407 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD90_RS12875 (K6D_25020)	56814..58298	+	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-
QUD90_RS12880 (K6D_25030)	58352..59155	+	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
QUD90_RS12885 (K6D_25040)	59464..60765	-	1302	Protein_64	ISL3 family transposase	-
QUD90_RS12890 (K6D_25050)	61183..61407	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
QUD90_RS12895 (K6D_25060)	61422..62291	+	870	WP_071661597.1	nucleotidyltransferase domain-containing protein	Toxin
QUD90_RS12900 (K6D_25070)	62272..63006	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
QUD90_RS12905	63039..63149	+	111	Protein_68	aminoglycoside 6-adenylyltransferase	-
QUD90_RS12910 (K6D_25080)	63168..63560	+	393	WP_000393259.1	GNAT family N-acetyltransferase	-
QUD90_RS12915 (K6D_25090)	63561..65000	+	1440	WP_001028144.1	aminoglycoside O-phosphotransferase APH(2'')-Ia	-
QUD90_RS12920 (K6D_25100)	65170..65967	+	798	Protein_71	aminoglycoside 6-adenylyltransferase	-
QUD90_RS12925 (K6D_25110)	65964..66506	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32902.55 Da
• Isoelectric Point: 4.9770

>T43696 WP_071661597.1 NZ_AP027295:61422-62291 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T43696 NZ_AP027295:61422-62291 [Enterococcus faecium]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTTGACTTGATTTTACGTGATATAAAACGGGTAGAACAAAT
AATCAAAGATACGGAGCAAGGAATTGTTACTGCCAATTATCAGACCGGGCATCCCCATGGTTATATAAGTGCAATGTATC
GTGGAGAATTAGCGATTAGTAAAATACTATATGCTAAGAACGAAAGCTTATGCGAATTAAAAAAGCAGGCAGAAATTTAC
CCTACTGCTCTAAAGAAAAGCTTGATGAACTTTTTTATATTTGAAGCTGAATTCTCTTTAATGTTTGTAAAAGCAAATGC
GGGGGTAGAGGATAAATATTATATTGCAGGCCATGTTTTTCGTATTATTTCATGCTTAAATCAAGTACTATTTGCATGTA
ATAATGCTTATTGTATCAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAACATAAACCTGAAAAATATACCGAG
AAGGTAAATCATATTTTTGAAGTACTCGGTATCTCACTTTTTGAATGCTACGATATGACCGAGAAACTTTATAAAGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT43696 WP_000205227.1 NZ_AP027295:61183-61407 [Enterococcus faecium]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT43696 NZ_AP027295:61183-61407 [Enterococcus faecium]
ATGACTACGGCAGAAATGATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: A0A3S8RBH6