Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2447963..2448534 Replicon chromosome
Accession NZ_AP027294
Organism Enterococcus faecium strain K6D

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag QUD90_RS12120 Protein ID WP_002286801.1
Coordinates 2448193..2448534 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag QUD90_RS12115 Protein ID WP_002323011.1
Coordinates 2447963..2448193 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUD90_RS12090 (K6D_23370) 2443203..2444534 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
QUD90_RS12095 (K6D_23380) 2444556..2445182 + 627 WP_002286816.1 cysteine hydrolase -
QUD90_RS12100 (K6D_23390) 2445365..2445946 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
QUD90_RS12105 (K6D_23400) 2446558..2447133 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
QUD90_RS12110 (K6D_23410) 2447338..2447676 - 339 WP_002286804.1 hypothetical protein -
QUD90_RS12115 (K6D_23420) 2447963..2448193 + 231 WP_002323011.1 hypothetical protein Antitoxin
QUD90_RS12120 (K6D_23430) 2448193..2448534 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
QUD90_RS12125 (K6D_23440) 2449384..2449569 + 186 WP_002304207.1 hypothetical protein -
QUD90_RS12130 (K6D_23460) 2450074..2450268 + 195 WP_002295146.1 hypothetical protein -
QUD90_RS12135 2450459..2450708 + 250 Protein_2334 transposase -
QUD90_RS12140 (K6D_23470) 2450952..2451782 - 831 WP_002286789.1 manganese catalase family protein -
QUD90_RS12145 (K6D_23480) 2451990..2453324 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T43695 WP_002286801.1 NZ_AP027294:2448193-2448534 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T43695 NZ_AP027294:2448193-2448534 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT43695 WP_002323011.1 NZ_AP027294:2447963-2448193 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT43695 NZ_AP027294:2447963-2448193 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References