Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 27474..28117 Replicon plasmid pTA8711-2
Accession NZ_AP027265
Organism Klebsiella pneumoniae strain TA8711

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag QUE17_RS27010 Protein ID WP_000754566.1
Coordinates 27701..28117 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag QUE17_RS27005 Protein ID WP_001261276.1
Coordinates 27474..27704 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE17_RS26970 (KPTA8711_52000) 22593..22862 + 270 WP_000339857.1 hypothetical protein -
QUE17_RS26975 (KPTA8711_52010) 23039..23905 - 867 WP_004118283.1 replication initiation protein -
QUE17_RS26980 24435..24539 + 105 WP_032409716.1 hypothetical protein -
QUE17_RS26985 (KPTA8711_52020) 24668..24925 + 258 WP_000764642.1 hypothetical protein -
QUE17_RS26990 (KPTA8711_52030) 24983..25759 - 777 WP_000015958.1 site-specific integrase -
QUE17_RS26995 (KPTA8711_52040) 25756..26499 - 744 WP_000129823.1 hypothetical protein -
QUE17_RS27000 (KPTA8711_52050) 26550..26900 - 351 WP_000493378.1 hypothetical protein -
QUE17_RS27005 (KPTA8711_52060) 27474..27704 + 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
QUE17_RS27010 (KPTA8711_52070) 27701..28117 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
QUE17_RS27015 28321..29177 + 857 Protein_35 IS3-like element ISEc15 family transposase -
QUE17_RS27020 (KPTA8711_52100) 29459..30457 + 999 Protein_36 IS110 family transposase -
QUE17_RS27025 (KPTA8711_52110) 30510..31214 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
QUE17_RS27030 (KPTA8711_52120) 31278..31886 - 609 Protein_38 DNA polymerase V subunit UmuC -
QUE17_RS27035 (KPTA8711_52130) 31886..32317 - 432 WP_000776034.1 translesion error-prone DNA polymerase V autoproteolytic subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / mph(A) - 1..39088 39088


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T43686 WP_000754566.1 NZ_AP027265:27701-28117 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T43686 NZ_AP027265:27701-28117 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT43686 WP_001261276.1 NZ_AP027265:27474-27704 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT43686 NZ_AP027265:27474-27704 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References