Detailed information of TA system TA043685

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 13939..14464
• Replicon: plasmid pTA8711-1
• Accession: NZ_AP027264
• Organism: Klebsiella pneumoniae strain TA8711

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: W8UQV6
• Locus tag: QUE17_RS26290
• Protein ID: WP_001568026.1
• Coordinates: 13939..14244 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: W8V2V6
• Locus tag: QUE17_RS26295
• Protein ID: WP_001568025.1
• Coordinates: 14246..14464 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE17_RS26270 (KPTA8711_50680)	10409..11677	+	1269	WP_019705993.1	ISL3-like element ISKpn25 family transposase	-
QUE17_RS26275 (KPTA8711_50690)	12012..13028	-	1017	WP_020315256.1	hypothetical protein	-
QUE17_RS26280 (KPTA8711_50700)	13025..13348	-	324	WP_022644730.1	hypothetical protein	-
QUE17_RS26285 (KPTA8711_50710)	13375..13770	-	396	WP_072158608.1	hypothetical protein	-
QUE17_RS26290 (KPTA8711_50720)	13939..14244	-	306	WP_001568026.1	type II toxin-antitoxin system toxin CcdB	Toxin
QUE17_RS26295 (KPTA8711_50730)	14246..14464	-	219	WP_001568025.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
QUE17_RS26300	14634..14879	-	246	Protein_13	hypothetical protein	-
QUE17_RS26305 (KPTA8711_50740)	15019..15708	+	690	WP_004193995.1	hypothetical protein	-
QUE17_RS26310 (KPTA8711_50750)	15740..16429	-	690	WP_020315560.1	RES family NAD+ phosphorylase	-
QUE17_RS26315 (KPTA8711_50760)	16866..17114	+	249	WP_000272716.1	hypothetical protein	-
QUE17_RS26320 (KPTA8711_50770)	17111..17683	+	573	WP_020805590.1	hypothetical protein	-
QUE17_RS26325 (KPTA8711_50780)	17714..18208	+	495	WP_020805591.1	hypothetical protein	-
QUE17_RS26330	18252..18620	+	369	WP_004197807.1	hypothetical protein	-
QUE17_RS26335 (KPTA8711_50790)	18654..18857	+	204	WP_004197808.1	HHA domain-containing protein	-
QUE17_RS26340 (KPTA8711_50800)	18871..19074	+	204	WP_004197809.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaKPC-2	-	1..106575	106575
	-	flank	IS/Tn	-	-	10409..11677	1268

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11645.32 Da
• Isoelectric Point: 5.6919

>T43685 WP_001568026.1 NZ_AP027264:c14244-13939 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASAWLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T43685 NZ_AP027264:c14244-13939 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCTGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8392.34 Da
• Isoelectric Point: 4.7405

>AT43685 WP_001568025.1 NZ_AP027264:c14464-14246 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT43685 NZ_AP027264:c14464-14246 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACTGTCACCATAGACAGTGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A514EZN1

Antitoxin

• AlphaFold DB: A0A2J4ZZP8