Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5241343..5241968 Replicon chromosome
Accession NZ_AP027263
Organism Klebsiella pneumoniae strain TA8711

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag QUE17_RS25870 Protein ID WP_002882817.1
Coordinates 5241343..5241726 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag QUE17_RS25875 Protein ID WP_004150355.1
Coordinates 5241726..5241968 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE17_RS25855 (KPTA8711_49900) 5238709..5239611 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
QUE17_RS25860 (KPTA8711_49910) 5239608..5240243 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
QUE17_RS25865 (KPTA8711_49920) 5240240..5241169 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
QUE17_RS25870 (KPTA8711_49930) 5241343..5241726 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
QUE17_RS25875 (KPTA8711_49940) 5241726..5241968 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
QUE17_RS25880 (KPTA8711_49950) 5242173..5243090 + 918 WP_002882812.1 alpha/beta hydrolase -
QUE17_RS25885 (KPTA8711_49960) 5243104..5244045 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
QUE17_RS25890 (KPTA8711_49970) 5244090..5244527 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
QUE17_RS25895 (KPTA8711_49980) 5244524..5245384 - 861 WP_002882807.1 virulence factor BrkB family protein -
QUE17_RS25900 (KPTA8711_49990) 5245378..5245977 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T43684 WP_002882817.1 NZ_AP027263:c5241726-5241343 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T43684 NZ_AP027263:c5241726-5241343 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT43684 WP_004150355.1 NZ_AP027263:c5241968-5241726 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT43684 NZ_AP027263:c5241968-5241726 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References