Detailed information of TA system TA043662

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 73778..74303
• Replicon: plasmid pJNE151685-1
• Accession: NZ_AP027259
• Organism: Escherichia coli strain JNE151685

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: QUE29_RS24690
• Protein ID: WP_001159871.1
• Coordinates: 73998..74303 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q3ZU16
• Locus tag: QUE29_RS24685
• Protein ID: WP_000813639.1
• Coordinates: 73778..73996 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE29_RS24650	69722..69939	+	218	Protein_62	hypothetical protein	-
QUE29_RS24655	69943..70053	+	111	Protein_63	IS481 family transposase	-
QUE29_RS24660 (JNE151685_46990)	70325..71056	+	732	WP_000850414.1	conjugal transfer complement resistance protein TraT	-
QUE29_RS24665 (JNE151685_47000)	71116..71487	+	372	WP_001373114.1	colicin-like pore-forming protein	-
QUE29_RS24670 (JNE151685_47030)	71970..72317	+	348	WP_000142427.1	DUF6404 family protein	-
QUE29_RS24675 (JNE151685_47040)	72335..72925	-	591	WP_033802802.1	hypothetical protein	-
QUE29_RS24680 (JNE151685_47050)	72922..73182	-	261	WP_000343100.1	hypothetical protein	-
QUE29_RS24685 (JNE151685_47070)	73778..73996	+	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
QUE29_RS24690 (JNE151685_47080)	73998..74303	+	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
QUE29_RS24695 (JNE151685_47090)	74304..75110	+	807	WP_000016966.1	site-specific integrase	-
QUE29_RS24700 (JNE151685_47100)	75290..75934	+	645	WP_001144036.1	ParA family protein	-
QUE29_RS24705 (JNE151685_47110)	76021..76329	+	309	WP_000030199.1	hypothetical protein	-
QUE29_RS24710 (JNE151685_47120)	76744..77724	+	981	WP_000688510.1	plasmid segregation protein ParM	-
QUE29_RS24715 (JNE151685_47130)	77717..78133	+	417	WP_001278809.1	plasmid partitioning/stability family protein	-
QUE29_RS24720 (JNE151685_47140)	78135..78308	-	174	Protein_76	DUF4113 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / hlyC / hlyA / hlyB / hlyD	1..173640	173640

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T43662 WP_001159871.1 NZ_AP027259:73998-74303 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T43662 NZ_AP027259:73998-74303 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.30 Da
• Isoelectric Point: 4.5203

>AT43662 WP_000813639.1 NZ_AP027259:73778-73996 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT43662 NZ_AP027259:73778-73996 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8

Antitoxin

• AlphaFold DB: A0A7U9DIR5