Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4903726..4904302 Replicon chromosome
Accession NZ_AP027258
Organism Escherichia coli strain JNE151685

Toxin (Protein)


Gene name shpA Uniprot ID A0A0E2LC92
Locus tag QUE29_RS24000 Protein ID WP_001299718.1
Coordinates 4903726..4904013 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A066STF6
Locus tag QUE29_RS24005 Protein ID WP_000063148.1
Coordinates 4904000..4904302 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE29_RS23985 (4899341) 4899341..4900690 - 1350 WP_115724179.1 restriction endonuclease subunit S -
QUE29_RS23990 (4900680) 4900680..4902299 - 1620 WP_181666839.1 class I SAM-dependent DNA methyltransferase -
QUE29_RS23995 (4902363) 4902363..4903529 - 1167 WP_286347622.1 restriction endonuclease -
QUE29_RS24000 (4903726) 4903726..4904013 + 288 WP_001299718.1 BrnT family toxin Toxin
QUE29_RS24005 (4904000) 4904000..4904302 + 303 WP_000063148.1 BrnA antitoxin family protein Antitoxin
QUE29_RS24010 (4904336) 4904336..4905292 - 957 WP_001297640.1 GTPase -
QUE29_RS24015 (4905303) 4905303..4905506 - 204 WP_000467859.1 YbdD/YjiX family protein -
QUE29_RS24020 (4905556) 4905556..4907706 - 2151 WP_001309197.1 pyruvate/proton symporter BtsT -
QUE29_RS24025 (4908099) 4908099..4908611 - 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11142.60 Da        Isoelectric Point: 7.4697

>T43661 WP_001299718.1 NZ_AP027258:4903726-4904013 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIENGEYRWQTIGVVHGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERSRYEHG

Download         Length: 288 bp

>T43661 NZ_AP027258:4903726-4904013 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTGGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCG
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGTCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11592.12 Da        Isoelectric Point: 9.4646

>AT43661 WP_000063148.1 NZ_AP027258:4904000-4904302 [Escherichia coli]
MSMVKHKRGTPSTLDVQHEAELKALANKFDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT43661 NZ_AP027258:4904000-4904302 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTTGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E2LC92

Antitoxin

Source ID Structure
AlphaFold DB A0A066STF6

References