Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3887772..3888403 Replicon chromosome
Accession NZ_AP027258
Organism Escherichia coli strain JNE151685

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag QUE29_RS19255 Protein ID WP_001259388.1
Coordinates 3888128..3888403 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag QUE29_RS19250 Protein ID WP_000593555.1
Coordinates 3887772..3888131 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE29_RS19225 (3883904) 3883904..3884848 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
QUE29_RS19230 (3884845) 3884845..3885678 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
QUE29_RS19235 (3885678) 3885678..3886442 + 765 WP_001136236.1 nickel import ATP-binding protein NikD -
QUE29_RS19240 (3886439) 3886439..3887245 + 807 WP_000173666.1 nickel import ATP-binding protein NikE -
QUE29_RS19245 (3887251) 3887251..3887652 + 402 WP_001190064.1 nickel-responsive transcriptional regulator NikR -
QUE29_RS19250 (3887772) 3887772..3888131 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QUE29_RS19255 (3888128) 3888128..3888403 - 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
QUE29_RS19260 (3888476) 3888476..3889600 - 1125 WP_001216257.1 ABC transporter permease -
QUE29_RS19265 (3889600) 3889600..3892335 - 2736 WP_182533492.1 ribosome-associated ATPase/putative transporter RbbA -
QUE29_RS19270 (3892332) 3892332..3893399 - 1068 WP_000361470.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T43657 WP_001259388.1 NZ_AP027258:c3888403-3888128 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T43657 NZ_AP027258:c3888403-3888128 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT43657 WP_000593555.1 NZ_AP027258:c3888131-3887772 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT43657 NZ_AP027258:c3888131-3887772 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References