Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3296724..3297378 Replicon chromosome
Accession NZ_AP027258
Organism Escherichia coli strain JNE151685

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag QUE29_RS16255 Protein ID WP_000244781.1
Coordinates 3296724..3297131 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUE29_RS16260 Protein ID WP_000354046.1
Coordinates 3297112..3297378 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE29_RS16235 (3292681) 3292681..3294414 - 1734 WP_000813202.1 single-stranded-DNA-specific exonuclease RecJ -
QUE29_RS16240 (3294420) 3294420..3295130 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUE29_RS16245 (3295155) 3295155..3296051 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUE29_RS16250 (3296163) 3296163..3296684 + 522 WP_001055865.1 flavodoxin FldB -
QUE29_RS16255 (3296724) 3296724..3297131 - 408 WP_000244781.1 protein YgfX Toxin
QUE29_RS16260 (3297112) 3297112..3297378 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUE29_RS16265 (3297621) 3297621..3298601 + 981 WP_182533433.1 tRNA-modifying protein YgfZ -
QUE29_RS16270 (3298678) 3298678..3299337 - 660 WP_000250274.1 hemolysin III family protein -
QUE29_RS16275 (3299501) 3299501..3299812 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
QUE29_RS16280 (3299857) 3299857..3301290 + 1434 WP_182533434.1 6-phospho-beta-glucosidase BglA -
QUE29_RS16285 (3301347) 3301347..3302090 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T43654 WP_000244781.1 NZ_AP027258:c3297131-3296724 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T43654 NZ_AP027258:c3297131-3296724 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATCTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTGCTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGTATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT43654 WP_000354046.1 NZ_AP027258:c3297378-3297112 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT43654 NZ_AP027258:c3297378-3297112 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References