Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 1610257..1610895 Replicon chromosome
Accession NZ_AP027258
Organism Escherichia coli strain JNE151685

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag QUE29_RS08010 Protein ID WP_000813794.1
Coordinates 1610257..1610433 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag QUE29_RS08015 Protein ID WP_001270286.1
Coordinates 1610479..1610895 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE29_RS07990 (1605876) 1605876..1607090 - 1215 WP_113073923.1 BenE family transporter YdcO -
QUE29_RS07995 (1607143) 1607143..1607679 + 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
QUE29_RS08000 (1607752) 1607752..1609713 + 1962 WP_001515270.1 23S rRNA 5-hydroxycytidine C2501 synthase -
QUE29_RS08005 (1609805) 1609805..1610035 - 231 WP_000494244.1 YncJ family protein -
QUE29_RS08010 (1610257) 1610257..1610433 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
QUE29_RS08015 (1610479) 1610479..1610895 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
QUE29_RS08020 (1610974) 1610974..1612380 + 1407 WP_000760629.1 PLP-dependent aminotransferase family protein -
QUE29_RS08025 (1612625) 1612625..1613770 + 1146 WP_000047456.1 ABC transporter substrate-binding protein -
QUE29_RS08030 (1613788) 1613788..1614801 + 1014 WP_000220411.1 ABC transporter ATP-binding protein -
QUE29_RS08035 (1614802) 1614802..1615744 + 943 Protein_1571 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T43641 WP_000813794.1 NZ_AP027258:1610257-1610433 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T43641 NZ_AP027258:1610257-1610433 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT43641 WP_001270286.1 NZ_AP027258:1610479-1610895 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT43641 NZ_AP027258:1610479-1610895 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References