Detailed information of TA system TA043637

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 446873..447710
• Replicon: chromosome
• Accession: NZ_AP027258
• Organism: Escherichia coli strain JNE151685

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QUE29_RS02080
• Protein ID: WP_000227784.1
• Coordinates: 446873..447415 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: QUE29_RS02085
• Protein ID: WP_001297137.1
• Coordinates: 447399..447710 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE29_RS02060 (442412)	442412..443323	-	912	WP_000705849.1	2-dehydropantoate 2-reductase	-
QUE29_RS02065 (443491)	443491..443982	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
QUE29_RS02070 (444110)	444110..445474	-	1365	WP_286347631.1	MFS transporter	-
QUE29_RS02075 (445882)	445882..446817	+	936	WP_001365761.1	tetratricopeptide repeat protein	-
QUE29_RS02080 (446873)	446873..447415	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QUE29_RS02085 (447399)	447399..447710	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
QUE29_RS02090 (447895)	447895..448785	-	891	WP_000971336.1	heme o synthase	-
QUE29_RS02095 (448797)	448797..449126	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QUE29_RS02100 (449126)	449126..449740	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QUE29_RS02105 (449730)	449730..451721	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QUE29_RS02110 (451743)	451743..452690	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T43637 WP_000227784.1 NZ_AP027258:c447415-446873 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T43637 NZ_AP027258:c447415-446873 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT43637 WP_001297137.1 NZ_AP027258:c447710-447399 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT43637 NZ_AP027258:c447710-447399 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3