Detailed information of TA system TA043636

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 70763..71288
• Replicon: plasmid pJNE141411-1
• Accession: NZ_AP027257
• Organism: Escherichia coli strain JNE141411

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: QUD91_RS25190
• Protein ID: WP_001159871.1
• Coordinates: 70983..71288 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q3ZU16
• Locus tag: QUD91_RS25185
• Protein ID: WP_000813639.1
• Coordinates: 70763..70981 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD91_RS25150	66707..66924	+	218	Protein_61	hypothetical protein	-
QUD91_RS25155	66928..67038	+	111	Protein_62	IS481 family transposase	-
QUD91_RS25160 (JNE141411_47910)	67310..68041	+	732	WP_000850414.1	conjugal transfer complement resistance protein TraT	-
QUD91_RS25165 (JNE141411_47920)	68101..68472	+	372	WP_001373114.1	colicin-like pore-forming protein	-
QUD91_RS25170 (JNE141411_47950)	68955..69302	+	348	WP_000142427.1	DUF6404 family protein	-
QUD91_RS25175 (JNE141411_47960)	69320..69910	-	591	WP_033802802.1	hypothetical protein	-
QUD91_RS25180 (JNE141411_47970)	69907..70167	-	261	WP_000343100.1	hypothetical protein	-
QUD91_RS25185 (JNE141411_47990)	70763..70981	+	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
QUD91_RS25190 (JNE141411_48000)	70983..71288	+	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
QUD91_RS25195 (JNE141411_48010)	71289..72095	+	807	WP_000016966.1	site-specific integrase	-
QUD91_RS25200 (JNE141411_48020)	72275..72919	+	645	WP_001144036.1	ParA family protein	-
QUD91_RS25205 (JNE141411_48030)	73006..73314	+	309	WP_000030199.1	hypothetical protein	-
QUD91_RS25210 (JNE141411_48040)	73729..74709	+	981	WP_000688510.1	plasmid segregation protein ParM	-
QUD91_RS25215 (JNE141411_48050)	74702..75118	+	417	WP_286335645.1	plasmid partitioning/stability family protein	-
QUD91_RS25220 (JNE141411_48060)	75120..75293	-	174	Protein_75	DUF4113 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / hlyC / hlyA / hlyB / hlyD	1..173999	173999

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T43636 WP_001159871.1 NZ_AP027257:70983-71288 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T43636 NZ_AP027257:70983-71288 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.30 Da
• Isoelectric Point: 4.5203

>AT43636 WP_000813639.1 NZ_AP027257:70763-70981 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT43636 NZ_AP027257:70763-70981 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8

Antitoxin

• AlphaFold DB: A0A7U9DIR5