Detailed information of TA system TA043630

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4169145..4169864
• Replicon: chromosome
• Accession: NZ_AP027256
• Organism: Escherichia coli strain JNE141411

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: E0J373
• Locus tag: QUD91_RS20525
• Protein ID: WP_001095907.1
• Coordinates: 4169544..4169864 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: E0J374
• Locus tag: QUD91_RS20520
• Protein ID: WP_001192122.1
• Coordinates: 4169145..4169510 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD91_RS20485 (4164239)	4164239..4164532	+	294	WP_000945828.1	hypothetical protein	-
QUD91_RS20495 (4165954)	4165954..4166757	+	804	WP_001245321.1	hypothetical protein	-
QUD91_RS20500 (4166974)	4166974..4167450	+	477	WP_001254693.1	hypothetical protein	-
QUD91_RS20505 (4167522)	4167522..4168343	+	822	WP_000197395.1	DUF932 domain-containing protein	-
QUD91_RS20510 (4168413)	4168413..4168883	+	471	WP_000104319.1	DNA repair protein RadC	-
QUD91_RS20515 (4168892)	4168892..4169113	+	222	WP_000691985.1	DUF987 family protein	-
QUD91_RS20520 (4169145)	4169145..4169510	+	366	WP_001192122.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
QUD91_RS20525 (4169544)	4169544..4169864	+	321	WP_001095907.1	TA system toxin CbtA family protein	Toxin
QUD91_RS20530 (4170073)	4170073..4170234	+	162	Protein_4021	RhuM family protein	-
QUD91_RS20535 (4170631)	4170631..4171815	+	1185	WP_001172876.1	sugar efflux transporter	-
QUD91_RS20540 (4171926)	4171926..4172849	+	924	WP_000535960.1	carboxylate/amino acid/amine transporter	-
QUD91_RS20545 (4172853)	4172853..4173671	-	819	WP_000779426.1	lipoprotein NlpA	-
QUD91_RS20550 (4173893)	4173893..4174186	+	294	WP_000805509.1	protein YicS	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (5-104)

Antitoxin

• Antitoxin CbeA (18-116)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12184.76 Da
• Isoelectric Point: 5.5740

>T43630 WP_001095907.1 NZ_AP027256:4169544-4169864 [Escherichia coli]
MNTPTVPATVTVSSRLSPVQVWQKLLTYILEHHYGLTINDTPFSNDTTIQEHIEAGVNLTDAVNFLVERFELVRIDQKGF
SWQDQEPWITSLDVRRAQFNLGLKRS

Nucleotide

• Length: 321 bp

>T43630 NZ_AP027256:4169544-4169864 [Escherichia coli]
ATGAATACTCCAACTGTACCGGCAACGGTGACGGTTTCGTCACGCCTGTCTCCTGTCCAAGTTTGGCAAAAACTATTAAC
GTATATTTTAGAGCATCATTACGGCCTCACGATCAACGACACACCGTTCAGTAACGATACGACGATTCAGGAACATATAG
AGGCTGGAGTGAATCTTACTGATGCTGTTAATTTTCTTGTGGAACGATTCGAGCTGGTGCGCATTGACCAGAAGGGTTTC
TCCTGGCAGGATCAAGAACCCTGGATAACATCTTTGGATGTTCGTCGTGCACAGTTCAACCTTGGCTTGAAACGCTCATA
A

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13470.22 Da
• Isoelectric Point: 6.8421

>AT43630 WP_001192122.1 NZ_AP027256:4169145-4169510 [Escherichia coli]
MQSTTLSGTSAENVSCLQWGLKRNITPCFGARLVQEGNRLYFLADRAGFNGSFSDDEALRLDQAFPLMMKQLERMLTAGE
LDPRSQHCVTRHHNGLTCEADTLGSHGYVYIAIYPQSALTR

Nucleotide

• Length: 366 bp

>AT43630 NZ_AP027256:4169145-4169510 [Escherichia coli]
ATGCAGTCGACCACCTTATCCGGCACATCGGCGGAGAACGTATCCTGCCTTCAGTGGGGTTTAAAACGTAATATCACGCC
ATGCTTCGGCGCTCGCCTGGTACAGGAAGGCAACCGCCTATACTTTCTGGCTGACCGGGCAGGATTTAATGGCTCATTCA
GTGACGATGAAGCTTTGCGTCTGGACCAGGCATTTCCACTGATGATGAAACAGCTTGAGCGGATGCTGACGGCTGGCGAG
CTTGATCCTCGCAGCCAGCACTGCGTCACACGGCATCACAACGGACTTACTTGTGAAGCTGACACTCTCGGCTCTCATGG
CTACGTTTACATTGCCATATATCCCCAGTCAGCTCTAACCAGATAA

Structures

Toxin

• AlphaFold DB: E0J373

Antitoxin

• AlphaFold DB: E0J374