Detailed information of TA system TA043613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 1595478..1596116
• Replicon: chromosome
• Accession: NZ_AP027256
• Organism: Escherichia coli strain JNE141411

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: QUD91_RS07810
• Protein ID: WP_000813794.1
• Coordinates: 1595478..1595654 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: QUD91_RS07815
• Protein ID: WP_001270285.1
• Coordinates: 1595700..1596116 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD91_RS07790 (1591097)	1591097..1592311	-	1215	WP_001326689.1	BenE family transporter YdcO	-
QUD91_RS07795 (1592364)	1592364..1592900	+	537	WP_000429153.1	DNA-binding transcriptional regulator SutR	-
QUD91_RS07800 (1592973)	1592973..1594934	+	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
QUD91_RS07805 (1595026)	1595026..1595256	-	231	WP_000494244.1	YncJ family protein	-
QUD91_RS07810 (1595478)	1595478..1595654	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
QUD91_RS07815 (1595700)	1595700..1596116	+	417	WP_001270285.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
QUD91_RS07820 (1596195)	1596195..1597601	+	1407	WP_000760589.1	PLP-dependent aminotransferase family protein	-
QUD91_RS07825 (1597846)	1597846..1598991	+	1146	WP_000034363.1	ABC transporter substrate-binding protein	-
QUD91_RS07830 (1599009)	1599009..1600022	+	1014	WP_000220411.1	ABC transporter ATP-binding protein	-
QUD91_RS07835 (1600023)	1600023..1600964	+	942	WP_001251313.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1589910..1590941	1031

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (88-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T43613 WP_000813794.1 NZ_AP027256:1595478-1595654 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T43613 NZ_AP027256:1595478-1595654 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15247.61 Da
• Isoelectric Point: 4.6115

>AT43613 WP_001270285.1 NZ_AP027256:1595700-1596116 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFDLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT43613 NZ_AP027256:1595700-1596116 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTGACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• PDB: 6HPB