Detailed information of TA system TA043611

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 468108..468945
• Replicon: chromosome
• Accession: NZ_AP027256
• Organism: Escherichia coli strain JNE141411

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: QUD91_RS02155
• Protein ID: WP_000227784.1
• Coordinates: 468108..468650 (-)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: QUD91_RS02160
• Protein ID: WP_001297137.1
• Coordinates: 468634..468945 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUD91_RS02135 (463647)	463647..464558	-	912	WP_000705874.1	2-dehydropantoate 2-reductase	-
QUD91_RS02140 (464726)	464726..465217	+	492	WP_001138904.1	nucleotide binding protein YajQ	-
QUD91_RS02145 (465345)	465345..466709	-	1365	WP_286335506.1	MFS transporter	-
QUD91_RS02150 (467117)	467117..468052	+	936	WP_001297127.1	tetratricopeptide repeat protein	-
QUD91_RS02155 (468108)	468108..468650	-	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
QUD91_RS02160 (468634)	468634..468945	-	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
QUD91_RS02165 (469130)	469130..470020	-	891	WP_000971336.1	heme o synthase	-
QUD91_RS02170 (470032)	470032..470361	-	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
QUD91_RS02175 (470361)	470361..470975	-	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
QUD91_RS02180 (470965)	470965..472956	-	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
QUD91_RS02185 (472978)	472978..473925	-	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T43611 WP_000227784.1 NZ_AP027256:c468650-468108 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T43611 NZ_AP027256:c468650-468108 [Escherichia coli]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT43611 WP_001297137.1 NZ_AP027256:c468945-468634 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT43611 NZ_AP027256:c468945-468634 [Escherichia coli]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
TGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3