Detailed information of TA system TA043609

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 68328..68853
• Replicon: plasmid pJNE120442-1
• Accession: NZ_AP027255
• Organism: Escherichia coli strain JNE120442

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: S1PFV8
• Locus tag: QUE31_RS24890
• Protein ID: WP_001159871.1
• Coordinates: 68548..68853 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A0F6YRL5
• Locus tag: QUE31_RS24885
• Protein ID: WP_038355956.1
• Coordinates: 68328..68546 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE31_RS24850	64459..64611	+	153	Protein_54	IS481 family transposase	-
QUE31_RS24855 (JNE120442_47390)	64888..65619	+	732	WP_000850413.1	conjugal transfer complement resistance protein TraT	-
QUE31_RS24860 (JNE120442_47400)	65735..66049	+	315	Protein_56	colicin-like pore-forming protein	-
QUE31_RS24865 (JNE120442_47410)	66056..66391	-	336	WP_021292998.1	colicin E1 family microcin immunity protein	-
QUE31_RS24870	66520..66867	+	348	WP_033817147.1	DUF6404 family protein	-
QUE31_RS24875 (JNE120442_47430)	66885..67475	-	591	WP_021293255.1	hypothetical protein	-
QUE31_RS24880 (JNE120442_47440)	67472..67732	-	261	WP_000343102.1	hypothetical protein	-
QUE31_RS24885 (JNE120442_47450)	68328..68546	+	219	WP_038355956.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
QUE31_RS24890 (JNE120442_47460)	68548..68853	+	306	WP_001159871.1	type II toxin-antitoxin system toxin CcdB	Toxin
QUE31_RS24895 (JNE120442_47470)	68854..69660	+	807	WP_040116953.1	site-specific integrase	-
QUE31_RS24900 (JNE120442_47480)	69840..70484	+	645	WP_001144036.1	ParA family protein	-
QUE31_RS24905 (JNE120442_47490)	70571..70879	+	309	WP_000030199.1	hypothetical protein	-
QUE31_RS24910 (JNE120442_47500)	71293..72273	+	981	WP_106912565.1	plasmid segregation protein ParM	-
QUE31_RS24915 (JNE120442_47510)	72266..72682	+	417	WP_001278818.1	plasmid partitioning/stability family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	espP / hlyC / hlyA / hlyB / hlyD	1..160552	160552

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11692.49 Da
• Isoelectric Point: 6.4674

>T43609 WP_001159871.1 NZ_AP027255:68548-68853 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T43609 NZ_AP027255:68548-68853 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTATCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8400.36 Da
• Isoelectric Point: 4.7405

>AT43609 WP_038355956.1 NZ_AP027255:68328-68546 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWRAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT43609 NZ_AP027255:68328-68546 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGACTGCGTGCTGAACGCTGGCGGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A829CCE8

Antitoxin

• AlphaFold DB: A0A0F6YRL5