Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4654634..4655131 Replicon chromosome
Accession NZ_AP027254
Organism Escherichia coli strain JNE120442

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUE31_RS22995 Protein ID WP_001173343.1
Coordinates 4654958..4655131 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID A0A1V2FLJ5
Locus tag QUE31_RS22990 Protein ID WP_001317561.1
Coordinates 4654634..4654930 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE31_RS22970 (4651365) 4651365..4652084 - 720 WP_000611288.1 two-component system response regulator DcuR -
QUE31_RS22975 (4652081) 4652081..4653712 - 1632 WP_001216482.1 sensor histidine kinase -
QUE31_RS22980 (4653893) 4653893..4654123 + 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
QUE31_RS22985 (4654135) 4654135..4654407 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
QUE31_RS22990 (4654634) 4654634..4654930 + 297 WP_001317561.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUE31_RS22995 (4654958) 4654958..4655131 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUE31_RS23000 (4655250) 4655250..4656767 - 1518 WP_286356065.1 lysine--tRNA ligase -
QUE31_RS23005 (4657004) 4657004..4658461 - 1458 WP_000856826.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T43608 WP_001173343.1 NZ_AP027254:4654958-4655131 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43608 NZ_AP027254:4654958-4655131 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11440.95 Da        Isoelectric Point: 4.1705

>AT43608 WP_001317561.1 NZ_AP027254:4654634-4654930 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMSFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43608 NZ_AP027254:4654634-4654930 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAGTTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB A0A1V2FLJ5

References