Detailed information of TA system TA043604

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3861685..3862316
• Replicon: chromosome
• Accession: NZ_AP027254
• Organism: Escherichia coli strain JNE120442

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: L4J012
• Locus tag: QUE31_RS19310
• Protein ID: WP_001260301.1
• Coordinates: 3862041..3862316 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IZA1
• Locus tag: QUE31_RS19305
• Protein ID: WP_000593557.1
• Coordinates: 3861685..3862044 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE31_RS19280 (3857817)	3857817..3858761	+	945	WP_000947063.1	nickel ABC transporter permease subunit NikB	-
QUE31_RS19285 (3858758)	3858758..3859591	+	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
QUE31_RS19290 (3859591)	3859591..3860355	+	765	WP_001136206.1	nickel import ATP-binding protein NikD	-
QUE31_RS19295 (3860352)	3860352..3861158	+	807	WP_000173650.1	nickel import ATP-binding protein NikE	-
QUE31_RS19300 (3861164)	3861164..3861565	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
QUE31_RS19305 (3861685)	3861685..3862044	-	360	WP_000593557.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
QUE31_RS19310 (3862041)	3862041..3862316	-	276	WP_001260301.1	type II toxin-antitoxin system HicA family toxin	Toxin
QUE31_RS19315 (3862389)	3862389..3863513	-	1125	WP_194882058.1	ABC transporter permease	-
QUE31_RS19320 (3863513)	3863513..3866248	-	2736	WP_052920229.1	ribosome-associated ATPase/putative transporter RbbA	-
QUE31_RS19325 (3866245)	3866245..3867312	-	1068	WP_000361470.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10185.87 Da
• Isoelectric Point: 10.6128

>T43604 WP_001260301.1 NZ_AP027254:c3862316-3862041 [Escherichia coli]
MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T43604 NZ_AP027254:c3862316-3862041 [Escherichia coli]
ATGAGAACCCAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAATCTCCTGTTCCTCAAGG
GATTAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTT
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13389.28 Da
• Isoelectric Point: 5.1987

>AT43604 WP_000593557.1 NZ_AP027254:c3862044-3861685 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLTNAAAQQQVSVNTYIIETLNERLNHL

Nucleotide

• Length: 360 bp

>AT43604 NZ_AP027254:c3862044-3861685 [Escherichia coli]
ATGATCAAACTGAAGACACCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGTATTCAGGGGCTGCAAAAAGAAGGGG
AGCTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACATTACGCTATCCAGAATCGCTGAGTGAACGCCTTACTAATGCGGCTGCGCAGCAACAAGTCTCTGTAAATACTTA
TATTATTGAAACGCTAAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: L4J012

Antitoxin

• AlphaFold DB: L4IZA1