Detailed information of TA system TA043603

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3834445..3835040
• Replicon: chromosome
• Accession: NZ_AP027254
• Organism: Escherichia coli strain JNE120442

Toxin (Protein)

• Gene name: doc
• Uniprot ID: D6JG31
• Locus tag: QUE31_RS19155
• Protein ID: WP_000155159.1
• Coordinates: 3834445..3834822 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: D6JG32
• Locus tag: QUE31_RS19160
• Protein ID: WP_000557315.1
• Coordinates: 3834819..3835040 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE31_RS19135 (3829943)	3829943..3831013	-	1071	WP_000907828.1	sn-glycerol 3-phosphate ABC transporter ATP binding protein UgpC	-
QUE31_RS19140 (3831015)	3831015..3831860	-	846	WP_000572164.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
QUE31_RS19145 (3831857)	3831857..3832744	-	888	WP_000099289.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
QUE31_RS19150 (3832920)	3832920..3834236	-	1317	WP_000803191.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
QUE31_RS19155 (3834445)	3834445..3834822	-	378	WP_000155159.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
QUE31_RS19160 (3834819)	3834819..3835040	-	222	WP_000557315.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
QUE31_RS19165 (3835128)	3835128..3835841	-	714	WP_286356052.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
QUE31_RS19170 (3835843)	3835843..3836610	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
QUE31_RS19175 (3836607)	3836607..3837884	-	1278	WP_000803825.1	branched chain amino acid ABC transporter permease LivM	-
QUE31_RS19180 (3837881)	3837881..3838807	-	927	WP_000003010.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
QUE31_RS19185 (3838855)	3838855..3839964	-	1110	WP_001318085.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3829203..3837884	8681

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (27-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-53)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13964.25 Da
• Isoelectric Point: 6.7263

>T43603 WP_000155159.1 NZ_AP027254:c3834822-3834445 [Escherichia coli]
MTIQFISAEEIIYFHDKLLSVTPGVTGMSDPGRAEALLYRVLNKYEYEGVSDLWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILAANPEFVELTVDVAAGKVSLEDIVTRLRQRGT

Nucleotide

• Length: 378 bp

>T43603 NZ_AP027254:c3834822-3834445 [Escherichia coli]
ATGACCATTCAGTTTATCTCAGCGGAGGAGATAATTTATTTCCACGATAAATTGCTTAGCGTTACACCAGGTGTAACAGG
AATGTCCGATCCAGGGCGAGCAGAAGCGCTTTTGTACCGGGTACTGAATAAGTATGAGTATGAAGGCGTTTCTGATCTTT
GGTTGCTGGCGGCGATGCATCTATTGGCAATCTCGCGCGGTCATATCTTTAATGATGGTAATAAGCGCACCGCACTGTTT
ATCACGCTATTATTCCTTAAACGTAACGGCATCATACTTGCTGCAAACCCAGAGTTTGTAGAATTGACGGTTGATGTGGC
GGCGGGAAAAGTATCGTTAGAAGATATCGTTACCCGCTTGCGTCAACGCGGTACCTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8103.24 Da
• Isoelectric Point: 6.2168

>AT43603 WP_000557315.1 NZ_AP027254:c3835040-3834819 [Escherichia coli]
MHTMNYSEARQNLALVLETAATGTPVTITRRGHKAAVIISAEEFERYQKAKLDAEFDAIMAIHGSEIKELADK

Nucleotide

• Length: 222 bp

>AT43603 NZ_AP027254:c3835040-3834819 [Escherichia coli]
ATGCATACCATGAACTACAGCGAAGCGCGGCAGAACCTTGCATTAGTTCTGGAAACAGCGGCGACAGGTACTCCGGTAAC
GATCACGAGGCGTGGGCATAAGGCAGCTGTGATTATCAGCGCAGAAGAGTTTGAACGTTACCAGAAGGCTAAACTGGACG
CAGAATTTGACGCCATCATGGCTATTCATGGCAGCGAAATTAAGGAGTTAGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: D6JG31

Antitoxin

• AlphaFold DB: D6JG32