Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3264334..3264988 Replicon chromosome
Accession NZ_AP027254
Organism Escherichia coli strain JNE120442

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag QUE31_RS16285 Protein ID WP_000244781.1
Coordinates 3264334..3264741 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUE31_RS16290 Protein ID WP_000354046.1
Coordinates 3264722..3264988 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE31_RS16265 (3260291) 3260291..3262024 - 1734 WP_000813191.1 single-stranded-DNA-specific exonuclease RecJ -
QUE31_RS16270 (3262030) 3262030..3262740 - 711 WP_000715230.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUE31_RS16275 (3262765) 3262765..3263661 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUE31_RS16280 (3263773) 3263773..3264294 + 522 WP_001055874.1 flavodoxin FldB -
QUE31_RS16285 (3264334) 3264334..3264741 - 408 WP_000244781.1 protein YgfX Toxin
QUE31_RS16290 (3264722) 3264722..3264988 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUE31_RS16295 (3265241) 3265241..3266221 + 981 WP_000886050.1 tRNA-modifying protein YgfZ -
QUE31_RS16300 (3266298) 3266298..3266957 - 660 WP_000250269.1 hemolysin III family protein -
QUE31_RS16305 (3267121) 3267121..3267432 - 312 WP_001182953.1 N(4)-acetylcytidine aminohydrolase -
QUE31_RS16310 (3267477) 3267477..3268910 + 1434 WP_001389468.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T43601 WP_000244781.1 NZ_AP027254:c3264741-3264334 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T43601 NZ_AP027254:c3264741-3264334 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT43601 WP_000354046.1 NZ_AP027254:c3264988-3264722 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT43601 NZ_AP027254:c3264988-3264722 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCAGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References