Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2819609..2820234 Replicon chromosome
Accession NZ_AP027254
Organism Escherichia coli strain JNE120442

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag QUE31_RS14295 Protein ID WP_000911329.1
Coordinates 2819609..2820007 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag QUE31_RS14300 Protein ID WP_000450524.1
Coordinates 2820007..2820234 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE31_RS14275 (2815516) 2815516..2815716 + 201 WP_000383836.1 YpfN family protein -
QUE31_RS14280 (2815797) 2815797..2816495 - 699 WP_000679819.1 esterase -
QUE31_RS14285 (2816569) 2816569..2818584 - 2016 WP_000829310.1 tRNA cytosine(34) acetyltransferase TmcA -
QUE31_RS14290 (2818599) 2818599..2819462 - 864 WP_042104743.1 neutral zinc metallopeptidase -
QUE31_RS14295 (2819609) 2819609..2820007 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
QUE31_RS14300 (2820007) 2820007..2820234 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
QUE31_RS14305 (2820391) 2820391..2821104 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
QUE31_RS14310 (2821317) 2821317..2822351 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
QUE31_RS14315 (2822368) 2822368..2823246 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
QUE31_RS14320 (2823392) 2823392..2823964 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
QUE31_RS14325 (2823964) 2823964..2824434 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T43600 WP_000911329.1 NZ_AP027254:c2820007-2819609 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T43600 NZ_AP027254:c2820007-2819609 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAACATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACT
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTAACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT43600 WP_000450524.1 NZ_AP027254:c2820234-2820007 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT43600 NZ_AP027254:c2820234-2820007 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAGGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References