Detailed information of TA system TA043587

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1168725..1169509
• Replicon: chromosome
• Accession: NZ_AP027254
• Organism: Escherichia coli strain JNE120442

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: QUE31_RS05665
• Protein ID: WP_000613624.1
• Coordinates: 1168725..1169219 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: B1LI61
• Locus tag: QUE31_RS05670
• Protein ID: WP_001110446.1
• Coordinates: 1169216..1169509 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE31_RS05645 (1163918)	1163918..1165015	+	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-
QUE31_RS05650 (1165015)	1165015..1165956	+	942	WP_001317765.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
QUE31_RS05655 (1166022)	1166022..1167665	+	1644	WP_042105957.1	flagellar hook-associated protein FlgK	-
QUE31_RS05660 (1167677)	1167677..1168630	+	954	WP_001212762.1	flagellar hook-associated protein FlgL	-
QUE31_RS05665 (1168725)	1168725..1169219	-	495	WP_000613624.1	GNAT family N-acetyltransferase	Toxin
QUE31_RS05670 (1169216)	1169216..1169509	-	294	WP_001110446.1	DUF1778 domain-containing protein	Antitoxin
QUE31_RS05675 (1169643)	1169643..1172828	-	3186	WP_000827395.1	ribonuclease E	-
QUE31_RS05680 (1173401)	1173401..1174360	+	960	WP_000846329.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (85-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18142.04 Da
• Isoelectric Point: 7.7444

>T43587 WP_000613624.1 NZ_AP027254:c1169219-1168725 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIDRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T43587 NZ_AP027254:c1169219-1168725 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGATCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11079.67 Da
• Isoelectric Point: 10.5423

>AT43587 WP_001110446.1 NZ_AP027254:c1169509-1169216 [Escherichia coli]
MPAANNIPARRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT43587 NZ_AP027254:c1169509-1169216 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAGCCAGGCGCGAATCTTTAAACTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTATCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A829G775