Detailed information of TA system TA043586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 921442..922147
• Replicon: chromosome
• Accession: NZ_AP027254
• Organism: Escherichia coli strain JNE120442

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: QUE31_RS04475
• Protein ID: WP_000539521.1
• Coordinates: 921761..922147 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: QUE31_RS04470
• Protein ID: WP_001280945.1
• Coordinates: 921442..921771 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE31_RS04455 (916599)	916599..917510	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
QUE31_RS04460 (917688)	917688..920036	+	2349	WP_042102082.1	EAL domain-containing protein	-
QUE31_RS04465 (920044)	920044..921372	+	1329	WP_000086919.1	GGDEF domain-containing protein	-
QUE31_RS04470 (921442)	921442..921771	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
QUE31_RS04475 (921761)	921761..922147	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUE31_RS04480 (922373)	922373..923698	-	1326	WP_000049378.1	30S ribosomal protein S12 methylthiotransferase RimO	-
QUE31_RS04485 (923911)	923911..924294	+	384	WP_000497137.1	biofilm formation regulator BssR	-
QUE31_RS04490 (924405)	924405..925520	+	1116	WP_000555041.1	aldose sugar dehydrogenase YliI	-
QUE31_RS04495 (925517)	925517..926143	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T43586 WP_000539521.1 NZ_AP027254:c922147-921761 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T43586 NZ_AP027254:c922147-921761 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT43586 WP_001280945.1 NZ_AP027254:c921771-921442 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT43586 NZ_AP027254:c921771-921442 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6