Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 523013..523631 Replicon chromosome
Accession NZ_AP027254
Organism Escherichia coli strain JNE120442

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag QUE31_RS02470 Protein ID WP_001291435.1
Coordinates 523013..523231 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag QUE31_RS02475 Protein ID WP_000344800.1
Coordinates 523257..523631 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE31_RS02435 (518302) 518302..518874 + 573 WP_032237088.1 YbaY family lipoprotein -
QUE31_RS02440 (518905) 518905..519216 - 312 WP_042104056.1 MGMT family protein -
QUE31_RS02450 (519595) 519595..519948 + 354 WP_000878140.1 DUF1428 domain-containing protein -
QUE31_RS02455 (519990) 519990..521540 - 1551 WP_042104058.1 cyclic-guanylate-specific phosphodiesterase PdeB -
QUE31_RS02460 (521704) 521704..522174 - 471 WP_000136192.1 YlaC family protein -
QUE31_RS02465 (522290) 522290..522841 - 552 WP_000102564.1 maltose O-acetyltransferase -
QUE31_RS02470 (523013) 523013..523231 - 219 WP_001291435.1 HHA domain-containing protein Toxin
QUE31_RS02475 (523257) 523257..523631 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
QUE31_RS02480 (524177) 524177..527326 - 3150 WP_001132480.1 efflux RND transporter permease AcrB -
QUE31_RS02485 (527349) 527349..528542 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T43584 WP_001291435.1 NZ_AP027254:c523231-523013 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T43584 NZ_AP027254:c523231-523013 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT43584 WP_000344800.1 NZ_AP027254:c523631-523257 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT43584 NZ_AP027254:c523631-523257 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCGCAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References