Detailed information of TA system TA043583

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 2238..2712
• Replicon: plasmid pJNE120393-2
• Accession: NZ_AP027248
• Organism: Escherichia coli strain JNE120393

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: QUF33_RS25880
• Protein ID: WP_286339058.1
• Coordinates: 2238..2504 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A7U2VUZ4
• Locus tag: QUF33_RS25885
• Protein ID: WP_000879770.1
• Coordinates: 2488..2712 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF33_RS25865 (JNE120393_49590)	1075..1365	+	291	WP_024200900.1	hypothetical protein	-
QUF33_RS25870 (JNE120393_49600)	1357..1635	-	279	WP_000710675.1	hypothetical protein	-
QUF33_RS25875 (JNE120393_49610)	1757..2179	+	423	WP_032319274.1	Exc2 family lipoprotein	-
QUF33_RS25880 (JNE120393_49620)	2238..2504	-	267	WP_286339058.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
QUF33_RS25885 (JNE120393_49630)	2488..2712	-	225	WP_000879770.1	DUF6290 family protein	Antitoxin
QUF33_RS25890 (JNE120393_49640)	2866..3078	+	213	WP_000350688.1	hypothetical protein	-
QUF33_RS25895 (JNE120393_49660)	3610..3843	-	234	WP_000137429.1	MbeD family mobilization/exclusion protein	-
QUF33_RS25900 (JNE120393_49670)	3850..4410	-	561	WP_001345151.1	MbeB family mobilization protein	-
QUF33_RS25905	4442..4879	-	438	Protein_9	relaxase/mobilization nuclease domain-containing protein	-
QUF33_RS25910 (JNE120393_49680)	5088..5411	-	324	WP_001444404.1	MobC family plasmid mobilization relaxosome protein	-
QUF33_RS25915 (JNE120393_49690)	5479..5670	+	192	WP_000165980.1	Rop family plasmid primer RNA-binding protein	-
QUF33_RS25920	6679..6810	-	132	WP_077521118.1	colicin release lysis protein	-
QUF33_RS25925	6840..7130	+	291	WP_011264161.1	colicin K immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..7769	7769

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10471.18 Da
• Isoelectric Point: 9.8720

>T43583 WP_286339058.1 NZ_AP027248:c2504-2238 [Escherichia coli]
MVWTINYSDRALKSLRKMDKQNARRIVDFMDLRIAVAADPRKSGRLLKGELGEFWRYRVGDYRILCEIRDDELIILVATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T43583 NZ_AP027248:c2504-2238 [Escherichia coli]
TTGGTCTGGACGATTAATTATTCCGATCGGGCGCTCAAATCGCTACGTAAGATGGACAAGCAGAACGCGCGACGGATTGT
GGATTTTATGGATTTACGTATTGCAGTTGCAGCCGATCCCCGTAAATCAGGGAGGCTACTGAAGGGGGAGTTGGGAGAAT
TCTGGCGTTATCGTGTGGGTGATTATCGCATTCTGTGTGAAATCAGGGATGACGAACTGATTATTCTTGTGGCCACCATC
GGCCATCGACGTGAAGTCTACGACTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8369.33 Da
• Isoelectric Point: 4.8552

>AT43583 WP_000879770.1 NZ_AP027248:c2712-2488 [Escherichia coli]
MLAIRLSDEIESRLDSLAKKTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEDVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT43583 NZ_AP027248:c2712-2488 [Escherichia coli]
ATGCTGGCTATTCGGTTGTCTGATGAAATTGAGTCCCGTCTGGACTCGCTGGCGAAGAAAACCGGGAGGACAAAGACGTT
CTATGCCCGGGAAGCGATACTGGCGCACCTGGAAGATCTGGAAGATTATTATCTTTCAGCGGAAACAGCAGCACGTGTTC
GCCGTGGTGATGAGGCTGTGCATTCGTCTGAGGACGTAAGGAAGTCTCTTGGTCTGGACGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U2VUZ4