Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 62895..63420 Replicon plasmid pJNE120393-1
Accession NZ_AP027247
Organism Escherichia coli strain JNE120393

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag QUF33_RS25320 Protein ID WP_001159871.1
Coordinates 63115..63420 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q3ZU16
Locus tag QUF33_RS25315 Protein ID WP_000813639.1
Coordinates 62895..63113 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF33_RS25285 59027..59179 + 153 Protein_50 IS481 family transposase -
QUF33_RS25290 (JNE120393_48400) 59456..60187 + 732 WP_000850413.1 conjugal transfer complement resistance protein TraT -
QUF33_RS25295 (JNE120393_48410) 60297..60617 + 321 WP_001365556.1 colicin-like pore-forming protein -
QUF33_RS25300 (JNE120393_48440) 61087..61434 + 348 WP_000142426.1 DUF6404 family protein -
QUF33_RS25305 (JNE120393_48450) 61452..62042 - 591 WP_000194569.1 hypothetical protein -
QUF33_RS25310 (JNE120393_48460) 62039..62299 - 261 WP_000343100.1 hypothetical protein -
QUF33_RS25315 (JNE120393_48480) 62895..63113 + 219 WP_000813639.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
QUF33_RS25320 (JNE120393_48490) 63115..63420 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
QUF33_RS25325 (JNE120393_48500) 63421..64227 + 807 WP_000016966.1 site-specific integrase -
QUF33_RS25330 (JNE120393_48510) 64407..65051 + 645 WP_001144036.1 ParA family protein -
QUF33_RS25335 (JNE120393_48520) 65138..65446 + 309 WP_000030199.1 hypothetical protein -
QUF33_RS25340 65861..66840 + 980 Protein_61 plasmid segregation protein ParM -
QUF33_RS25345 (JNE120393_48550) 66833..67249 + 417 WP_001278809.1 plasmid partitioning/stability family protein -
QUF33_RS25350 (JNE120393_48560) 67251..67424 - 174 Protein_63 DUF4113 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - espP / hlyC / hlyA / hlyB / hlyD 1..160690 160690


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T43582 WP_001159871.1 NZ_AP027247:63115-63420 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T43582 NZ_AP027247:63115-63420 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.30 Da        Isoelectric Point: 4.5203

>AT43582 WP_000813639.1 NZ_AP027247:62895-63113 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT43582 NZ_AP027247:62895-63113 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9DIR5

References