Detailed information of TA system TA043581

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4654816..4655441
• Replicon: chromosome
• Accession: NZ_AP027246
• Organism: Escherichia coli strain JNE120393

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: QUF33_RS23115
• Protein ID: WP_000911330.1
• Coordinates: 4655043..4655441 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: QUF33_RS23110
• Protein ID: WP_000450524.1
• Coordinates: 4654816..4655043 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUF33_RS23085 (4650619)	4650619..4651089	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
QUF33_RS23090 (4651089)	4651089..4651661	-	573	WP_000176191.1	glycine cleavage system transcriptional repressor	-
QUF33_RS23095 (4651807)	4651807..4652685	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
QUF33_RS23100 (4652702)	4652702..4653736	+	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
QUF33_RS23105 (4653949)	4653949..4654662	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
QUF33_RS23110 (4654816)	4654816..4655043	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
QUF33_RS23115 (4655043)	4655043..4655441	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
QUF33_RS23120 (4655588)	4655588..4656451	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
QUF33_RS23125 (4656466)	4656466..4658481	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
QUF33_RS23130 (4658555)	4658555..4659253	+	699	WP_000679807.1	esterase	-
QUF33_RS23135 (4659363)	4659363..4659563	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T43581 WP_000911330.1 NZ_AP027246:4655043-4655441 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T43581 NZ_AP027246:4655043-4655441 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT43581 WP_000450524.1 NZ_AP027246:4654816-4655043 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT43581 NZ_AP027246:4654816-4655043 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33