Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 4219941..4220595 Replicon chromosome
Accession NZ_AP027246
Organism Escherichia coli strain JNE120393

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag QUF33_RS21045 Protein ID WP_000244772.1
Coordinates 4220188..4220595 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag QUF33_RS21040 Protein ID WP_000354046.1
Coordinates 4219941..4220207 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF33_RS21015 (4215229) 4215229..4215972 + 744 WP_000951964.1 SDR family oxidoreductase -
QUF33_RS21020 (4216029) 4216029..4217462 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
QUF33_RS21025 (4217507) 4217507..4217818 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
QUF33_RS21030 (4217982) 4217982..4218641 + 660 WP_000250274.1 hemolysin III family protein -
QUF33_RS21035 (4218718) 4218718..4219698 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
QUF33_RS21040 (4219941) 4219941..4220207 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
QUF33_RS21045 (4220188) 4220188..4220595 + 408 WP_000244772.1 protein YgfX Toxin
QUF33_RS21050 (4220635) 4220635..4221156 - 522 WP_001055874.1 flavodoxin FldB -
QUF33_RS21055 (4221268) 4221268..4222164 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
QUF33_RS21060 (4222189) 4222189..4222899 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
QUF33_RS21065 (4222905) 4222905..4224638 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T43579 WP_000244772.1 NZ_AP027246:4220188-4220595 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T43579 NZ_AP027246:4220188-4220595 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT43579 WP_000354046.1 NZ_AP027246:4219941-4220207 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT43579 NZ_AP027246:4219941-4220207 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References