Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 3537255..3537841 Replicon chromosome
Accession NZ_AP027246
Organism Escherichia coli strain JNE120393

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H0RQZ4
Locus tag QUF33_RS17605 Protein ID WP_042014162.1
Coordinates 3537255..3537485 (+) Length 77 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag QUF33_RS17610 Protein ID WP_000593555.1
Coordinates 3537482..3537841 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF33_RS17595 (3533292) 3533292..3536027 + 2736 WP_000149132.1 ribosome-associated ATPase/putative transporter RbbA -
QUF33_RS17600 (3536027) 3536027..3537151 + 1125 WP_001314210.1 ABC transporter permease -
QUF33_RS17605 (3537255) 3537255..3537485 + 231 WP_042014162.1 type II toxin-antitoxin system HicA family toxin Toxin
QUF33_RS17610 (3537482) 3537482..3537841 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
QUF33_RS17615 (3537961) 3537961..3538362 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
QUF33_RS17620 (3538368) 3538368..3539174 - 807 WP_000173666.1 nickel import ATP-binding protein NikE -
QUF33_RS17625 (3539171) 3539171..3539935 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
QUF33_RS17630 (3539935) 3539935..3540768 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
QUF33_RS17635 (3540765) 3540765..3541709 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(15-65)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 77 a.a.        Molecular weight: 8419.79 Da        Isoelectric Point: 8.5518

>T43575 WP_042014162.1 NZ_AP027246:3537255-3537485 [Escherichia coli]
MDQLFKSPLPQGIKWTDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP

Download         Length: 231 bp

>T43575 NZ_AP027246:3537255-3537485 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT43575 WP_000593555.1 NZ_AP027246:3537482-3537841 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT43575 NZ_AP027246:3537482-3537841 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0RQZ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References