Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2761773..2762270 Replicon chromosome
Accession NZ_AP027246
Organism Escherichia coli strain JNE120393

Toxin (Protein)


Gene name ghoT Uniprot ID -
Locus tag QUF33_RS14010 Protein ID WP_001173344.1
Coordinates 2761773..2761946 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUF33_RS14015 Protein ID WP_000398619.1
Coordinates 2761974..2762270 (-) Length 99 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF33_RS14000 (2758443) 2758443..2759900 + 1458 WP_000856832.1 dipeptide/tripeptide permease DtpC -
QUF33_RS14005 (2760137) 2760137..2761654 + 1518 WP_001295074.1 lysine--tRNA ligase -
QUF33_RS14010 (2761773) 2761773..2761946 - 174 WP_001173344.1 type V toxin-antitoxin system toxin GhoT Toxin
QUF33_RS14015 (2761974) 2761974..2762270 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUF33_RS14020 (2762497) 2762497..2762769 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
QUF33_RS14025 (2762781) 2762781..2763011 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QUF33_RS14030 (2763192) 2763192..2764823 + 1632 WP_001216461.1 sensor histidine kinase -
QUF33_RS14035 (2764820) 2764820..2765539 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(4-57)

Antitoxin

(5-94)


Sequences


Toxin        


Download         Length: 58 a.a.        Molecular weight: 6583.12 Da        Isoelectric Point: 10.2288

>T43573 WP_001173344.1 NZ_AP027246:c2761946-2761773 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSVFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43573 NZ_AP027246:c2761946-2761773 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGTATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT43573 WP_000398619.1 NZ_AP027246:c2762270-2761974 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43573 NZ_AP027246:c2762270-2761974 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References