Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 2657986..2658581 Replicon chromosome
Accession NZ_AP027246
Organism Escherichia coli strain JNE120393

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag QUF33_RS13490 Protein ID WP_000239579.1
Coordinates 2657986..2658336 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag QUF33_RS13495 Protein ID WP_001223208.1
Coordinates 2658330..2658581 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUF33_RS13470 (2653262) 2653262..2654284 - 1023 WP_001339490.1 ABC transporter permease -
QUF33_RS13475 (2654298) 2654298..2655800 - 1503 WP_000205793.1 sugar ABC transporter ATP-binding protein -
QUF33_RS13480 (2656110) 2656110..2657066 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
QUF33_RS13485 (2657376) 2657376..2657906 + 531 WP_000055072.1 inorganic diphosphatase -
QUF33_RS13490 (2657986) 2657986..2658336 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
QUF33_RS13495 (2658330) 2658330..2658581 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
QUF33_RS13500 (2658794) 2658794..2659135 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
QUF33_RS13505 (2659138) 2659138..2662917 - 3780 WP_000060901.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T43572 WP_000239579.1 NZ_AP027246:c2658336-2657986 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T43572 NZ_AP027246:c2658336-2657986 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT43572 WP_001223208.1 NZ_AP027246:c2658581-2658330 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT43572 NZ_AP027246:c2658581-2658330 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References