Detailed information of TA system TA043556

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 153780..154305
• Replicon: plasmid pJNE170426-1
• Accession: NZ_AP027218
• Organism: Escherichia coli strain JNE170426

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A0F6YRT4
• Locus tag: QUE10_RS25300
• Protein ID: WP_021293254.1
• Coordinates: 153780..154085 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q3ZU16
• Locus tag: QUE10_RS25305
• Protein ID: WP_000813639.1
• Coordinates: 154087..154305 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE10_RS25270	149774..149947	+	174	Protein_148	DUF4113 domain-containing protein	-
QUE10_RS25275 (JNE170426_p11430)	149949..150365	-	417	WP_001278818.1	plasmid partitioning/stability family protein	-
QUE10_RS25280 (JNE170426_p11440)	150358..151338	-	981	WP_000688510.1	plasmid segregation protein ParM	-
QUE10_RS25285 (JNE170426_p11450)	151753..152061	-	309	WP_000030199.1	hypothetical protein	-
QUE10_RS25290 (JNE170426_p11460)	152148..152792	-	645	WP_001144036.1	ParA family protein	-
QUE10_RS25295 (JNE170426_p11470)	152973..153779	-	807	WP_000016966.1	site-specific integrase	-
QUE10_RS25300 (JNE170426_p11480)	153780..154085	-	306	WP_021293254.1	type II toxin-antitoxin system toxin CcdB	Toxin
QUE10_RS25305 (JNE170426_p11490)	154087..154305	-	219	WP_000813639.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
QUE10_RS25310 (JNE170426_p11500)	154901..155161	+	261	WP_000343102.1	hypothetical protein	-
QUE10_RS25315 (JNE170426_p11510)	155158..155748	+	591	WP_021293255.1	hypothetical protein	-
QUE10_RS25320	155766..156113	-	348	WP_033817147.1	DUF6404 family protein	-
QUE10_RS25325 (JNE170426_p11530)	156242..156577	+	336	WP_021292998.1	colicin E1 family microcin immunity protein	-
QUE10_RS25330 (JNE170426_p11540)	156584..156898	-	315	Protein_160	colicin-like pore-forming protein	-
QUE10_RS25335 (JNE170426_p11550)	157014..157745	-	732	WP_000850413.1	conjugal transfer complement resistance protein TraT	-
QUE10_RS25340	158022..158174	-	153	Protein_162	IS481 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	hlyD / hlyB / hlyA / hlyC / espP	1..158915	158915

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11634.45 Da
• Isoelectric Point: 7.2010

>T43556 WP_021293254.1 NZ_AP027218:c154085-153780 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSGIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T43556 NZ_AP027218:c154085-153780 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGGCATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGCGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCCGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.30 Da
• Isoelectric Point: 4.5203

>AT43556 WP_000813639.1 NZ_AP027218:c154305-154087 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT43556 NZ_AP027218:c154305-154087 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGCTGCTCAAGGCGTATGACGTGAATATCTCCGGTCT
GGTCAGTACGACCATGCAGAACGAAGCCCGGCGCCTGCGTGCTGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCAG
AGGTCGCCAGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A0F6YRT4

Antitoxin

• AlphaFold DB: A0A7U9DIR5