Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4703791..4704288 Replicon chromosome
Accession NZ_AP027217
Organism Escherichia coli strain JNE170426

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag QUE10_RS22850 Protein ID WP_001173343.1
Coordinates 4704115..4704288 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag QUE10_RS22845 Protein ID WP_000398619.1
Coordinates 4703791..4704087 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
QUE10_RS22825 (4700522) 4700522..4701241 - 720 WP_000611288.1 two-component system response regulator DcuR -
QUE10_RS22830 (4701238) 4701238..4702869 - 1632 WP_001216461.1 sensor histidine kinase -
QUE10_RS22835 (4703050) 4703050..4703280 + 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
QUE10_RS22840 (4703292) 4703292..4703564 + 273 WP_000405647.1 GNAT family N-acetyltransferase -
QUE10_RS22845 (4703791) 4703791..4704087 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
QUE10_RS22850 (4704115) 4704115..4704288 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
QUE10_RS22855 (4704407) 4704407..4705924 - 1518 WP_001295074.1 lysine--tRNA ligase -
QUE10_RS22860 (4706161) 4706161..4707618 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T43553 WP_001173343.1 NZ_AP027217:4704115-4704288 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T43553 NZ_AP027217:4704115-4704288 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT43553 WP_000398619.1 NZ_AP027217:4703791-4704087 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT43553 NZ_AP027217:4703791-4704087 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References