Detailed information of TA system TA043550

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 3904342..3904973
• Replicon: chromosome
• Accession: NZ_AP027217
• Organism: Escherichia coli strain JNE170426

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: F4NNK6
• Locus tag: QUE10_RS19180
• Protein ID: WP_001259388.1
• Coordinates: 3904698..3904973 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A8S7TCM0
• Locus tag: QUE10_RS19175
• Protein ID: WP_000593556.1
• Coordinates: 3904342..3904701 (-)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
QUE10_RS19150 (3900474)	3900474..3901418	+	945	WP_000947068.1	nickel ABC transporter permease subunit NikB	-
QUE10_RS19155 (3901415)	3901415..3902248	+	834	WP_001008963.1	nickel ABC transporter permease subunit NikC	-
QUE10_RS19160 (3902248)	3902248..3903012	+	765	WP_089601759.1	nickel import ATP-binding protein NikD	-
QUE10_RS19165 (3903009)	3903009..3903815	+	807	WP_000173666.1	nickel import ATP-binding protein NikE	-
QUE10_RS19170 (3903821)	3903821..3904222	+	402	WP_001190062.1	nickel-responsive transcriptional regulator NikR	-
QUE10_RS19175 (3904342)	3904342..3904701	-	360	WP_000593556.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
QUE10_RS19180 (3904698)	3904698..3904973	-	276	WP_001259388.1	type II toxin-antitoxin system HicA family toxin	Toxin
QUE10_RS19185 (3905046)	3905046..3906170	-	1125	WP_001314210.1	ABC transporter permease	-
QUE10_RS19190 (3906170)	3906170..3908905	-	2736	WP_000149125.1	ribosome-associated ATPase/putative transporter RbbA	-
QUE10_RS19195 (3908902)	3908902..3909969	-	1068	WP_000361485.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (30-80)

Antitoxin

• Uncharacterised protein family HicB (60-112)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10151.89 Da
• Isoelectric Point: 10.7076

>T43550 WP_001259388.1 NZ_AP027217:c3904973-3904698 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Nucleotide

• Length: 276 bp

>T43550 NZ_AP027217:c3904973-3904698 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13388.25 Da
• Isoelectric Point: 5.1987

>AT43550 WP_000593556.1 NZ_AP027217:c3904701-3904342 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHV

Nucleotide

• Length: 360 bp

>AT43550 NZ_AP027217:c3904701-3904342 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATGTATAA

Structures

Toxin

• AlphaFold DB: A0A0A0FFA2